Resonance Raman Studies of Electron-nuclear Coupling, Time Resolved Dynamics and Magnetic Perturbations of Biomolecules

生物分子电子-核耦合、时间分辨动力学和磁扰动的共振拉曼研究

• 批准号: 9016860
• 负责人: Paul Champion
• 金额: $ 29.4万
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-15 至 1994-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9016860&HistoricalAwards=false
• 关键词: Resonance; Raman; Studies; Electron; nuclear

The proposed research utilizes resonance Raman and Rayleigh scattering to probe the electronic and nuclear structure/function relationships of biological molecules such as the heme proteins (myoglobin, hemoglobin and cytochrome c). Related techniques, such as electronic absorption and far infrared spectroscopy, are also utilized and theoretical relationship that link a variety of experimental measurements are developed. Further experimental and theoretical studies involving the spectroscopic and kinetic effects of protein conformational distributions are also proposed. Dynamic studies will include the measurement and analysis of ligand binding to heme proteins under a wide variety of sample conditions as well as pump/probe experiments designed to monitor protein folding and conformational relaxation. Laser mediated thermal perturbations will be followed using Stokes/anti-Stokes Raman scattering. Studies of photon induced electron transport kinetics as well as resonance Raman saturation studies are also proposed. Resonance Raman and kinetic studies of biological Single crystals will also be extended. Overall the objectives are to achieve a better understanding of the resonant light scattering properties of biological molecules and to apply this knowledge to the elucidation of their mechanism of action. These studies are significant not only in their relationship to the biological sciences, but also in their connection to the understanding of basic light-matter interactions.

该研究利用共振拉曼和瑞利散射 散射探测电子和核 生物分子的结构/功能关系， 血红素蛋白（肌红蛋白、血红蛋白和细胞色素c）。 相关技术，如电子吸收和远 红外光谱，也被利用和理论 将各种实验测量联系起来的关系 是发达的。 进一步的实验和理论研究 包括蛋白质的光谱和动力学效应 还提出了构象分布。 动态 研究将包括配体的测量和分析 在多种样品下与血红素蛋白结合 条件以及泵/探针实验， 监测蛋白质折叠和构象松弛。 激光 介导的热扰动将采用 斯托克斯/反斯托克斯拉曼散射。 光子诱导的研究 电子输运动力学以及共振拉曼 还提出了饱和度研究。 共振拉曼和 生物单晶的动力学研究也将 延长 总的来说，目标是更好地了解 生物分子的共振光散射特性 分子，并将这些知识应用于阐明 他们的行动机制。 这些研究意义重大， 不仅在它们与生物科学的关系上， 与理解基本光物质的联系 交互.