Resonance Raman Studies of Electron-Nuclear Coupling, Time Resolved Dynamics, and Magnetic Perturbations of Biomolecules

电子-核耦合、时间分辨动力学和生物分子磁扰动的共振拉曼研究

• 批准号: 9405979
• 负责人: Paul Champion
• 金额: $ 62.71万
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9405979&HistoricalAwards=false
• 关键词: Resonance; Raman; Studies; Electron; Nuclear

9405979 Champion The proposed research will utilize resonance Raman scattering and its time domain analog, coherent impulsive scattering, to probe the electronic and nuclear structure/function relationships of biological molecules such as heme proteins (myoglobin, hemoglobin and cytochrome c) and photosynthetic systems (light harvesting antennae and reaction centers). Related techniques, such as electronic absorption and infrared spectroscopy, will also be utilized, and theoretical models that link a variety of experimental measurements will be developed. Absolute cross- section measurements will be used to directly extract the electron-nuclear coupling strengths while the temperature dependence of the optical absorption lineshape yields the coupling of low frequency motion and its role in damping. The studies of low frequency protein motion will be complemented with ultrafast impulsive scattering techniques, which allow real time measurement of such motion. Further experimental and theoretical studies involving the spectroscopic and kinetic effects of protein distributions are also proposed. Dynamic studies will include the measurement and analysis of ligand binding and electron transfer under a wide variety of sample conditions, as well as pump/probe experiments designed to monitor protein folding and conformational relaxations. Laser mediated thermal perturbations will be followed using Stokes/anti-Stokes Raman scattering. Studies of dynamic processes induced by photoreduction and kinetic studies of biological single crystals will also be extended. %%% The general scientific objectives are to achieve a better understanding of the dynamics of motion of large biologically important molecules and their absorption and resonant light scattering properties. The aim is to apply this knowledge to elucidate their mechanism of action. These studies are significant not only in their relationship to the biological sciences, but also in their connection to our u nderstanding of the interaction of light with complex material systems in the condensed phase. ***

9405979冠军拟议的研究将利用共振拉曼散射及其时域模拟，相干脉冲散射，探测生物分子的电子和核结构/功能关系，如血红素蛋白（肌红蛋白，血红蛋白和细胞色素c）和光合系统（光捕获天线和反应中心）。 还将利用电子吸收和红外光谱等相关技术，并将开发将各种实验测量联系起来的理论模型。 绝对截面测量将用于直接提取电子-核耦合强度，而光学吸收线形的温度依赖性产生低频运动的耦合及其在阻尼中的作用。 低频蛋白质运动的研究将补充超快脉冲散射技术，使这种运动的真实的时间测量。 进一步的实验和理论研究涉及蛋白质分布的光谱和动力学效应也提出了建议。 动态研究将包括各种样品条件下配体结合和电子转移的测量和分析，以及旨在监测蛋白质折叠和构象弛豫的泵/探针实验。 将使用斯托克斯/反斯托克斯拉曼散射跟踪激光介导的热扰动。 还将扩展光还原引起的动态过程的研究和生物单晶的动力学研究。 一般的科学目标是实现更好地了解大的生物重要分子的运动动力学及其吸收和共振光散射特性。 目的是应用这些知识来阐明其作用机制。 这些研究不仅在它们与生物科学的关系上具有重要意义，而且在它们与我们理解光与凝聚相中复杂物质系统的相互作用方面也具有重要意义。 ***