ABR: Experiments in Deep-Sea Microbiology

这项研究将解决这样一个问题：负责深海成矿过程的微生物的生理特征是什么？更具体地说：深海细菌对温度和压力的适应随着营养的变化而不变吗？将使用已知的耐压(耐压)生物种群中的菌株，以及新获得的深海分离株。生长实验将进行：1.作为连续培养实验，在大气压下，在原位底物浓度和温度范围为2-20℃；和2，作为分批培养，在原位压力下，再次在2-20℃和营养底物在实验上可能达到的最低浓度。在实验条件下，种群密度(细胞数和生物量)和/或放射性底物周转率的变化将被用作生长速度对各种可控环境因素：温度、压力和底物浓度变化的敏感指标。这项研究可能会为控制深海环境中微生物生长的因素和微生物转化物质的速度提供新的见解。

This study will address the question: What are the physiological characteristics of those microorganisms that are responsible for the mineralization processes in the deep sea? And more specifically: Are the temperature and pressure adaptations of deep-sea bacteria invariable with nutrition? Strains from a stock of known barophilic (pressure tolerant) organisms, as well as freshly-obtained deep sea isolates, will be used. Growth experiments will be carried out: 1. as continuous culture experiments at atmospheric pressure, and at in situ substrate concentrations and a temperature range of 2-20 oC; and 2. as batch cultures at in situ pressures, and temperatures again of 2- 20 oC and nutrient substrates at the experimentally lowest possible concentrations attainable. Under experimental conditions, changes in population density (cell counts and biomass) and/or radioactive substrate turnover will be used as a sensitive indicator of growth rate response to changes of the various controllable environmental factors: temperature, pressure and substrate concentration. This study potentially will provide new insights into factors regulating microbial growth and the rates of microbial transformations of matter in deep sea environments.