Mass Spectrometry: Instrument Development and Applications to Biomolecules

质谱分析：仪器开发及其在生物分子中的应用

• 批准号: 9017262
• 负责人: Michael Gross
• 金额: $ 46.73万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-15 至 1994-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9017262&HistoricalAwards=false
• 关键词: Mass; Spectrometry; Instrument; Development; Applications

This award provides funds to the University of Nebraska for funding of a facility that provides advanced mass spectrometric techniques through collaborations with researchers at Nebraska and at other institutions. One of several approaches to mass spectrometry (MS), tandem MS provides high resolution measurements of compounds with the high molecular weights typical of proteins, nucleic acids and other biopolymers. Efforts sponsored by this award will focus on improving techniques for analysis of variety of proteins, carbohydrates and other natural products using the tandem instrument; a variety of collaborative research projects will be initiated or continued, and training activities at the pre- and post doctoral level will continue. Mass spectrometry plays an increasingly important role in the structural analysis of biomolecules. Information about protein sequence and structure, including naturally occurring modifications of proteins, and similar kinds of information about polysaccharides and other branched chain polymers are obtained with great difficulty by other techniques. Because of the considerable expense of acquisition and operation of tandem MS instruments, this type of mass spectrometric analysis is not routinely available at most research institutions. The Nebraska facility is one of only several in the country that provide researchers at numerous institutions with access to the kind of skilled mass spectrometry needed in many areas of modern biological research.

该奖项为内布拉斯加大学提供资金， 资助一个设施，提供先进的质谱 通过与内布拉斯加州的研究人员合作， 和其他机构。 实现大规模的几种方法之一 串联质谱（MS）提供高分辨率 具有高分子量的化合物的测量 蛋白质、核酸和其他生物聚合物。 努力 由该奖项赞助的将专注于提高技术， 分析各种蛋白质，碳水化合物和其他天然 产品使用串联仪器;各种协作 将启动或继续开展研究项目， 将继续开展博士前和博士后的活动。 质谱分析在生物医学中起着越来越重要的作用。 生物分子结构分析。 蛋白质信息 序列和结构，包括天然存在的 蛋白质的修饰，以及类似的信息， 得到多糖和其它支链聚合物 通过其他技术非常困难。 因为 购置和操作串联质谱仪的费用相当高 仪器，这种类型的质谱分析不是 在大多数研究机构都可以得到。 内布拉斯加 该设施是该国仅有的几个提供 许多机构的研究人员都有机会接触到 现代工业的许多领域都需要熟练的质谱分析技术。 生物学研究