Phenolic/Signal Receptor Interactions in Agrobacterium

本研究将研究酚类化合物 与特定受体相互作用以引发细胞反应。 将酚类物质作为信号提示的一个重要模型系统是 根癌农杆菌是一种致病性土壤细菌， 在受感染植物的伤口部位引起肿瘤。 细菌 能将其Ti质粒上的DNA（T-DNA）导入植物细胞 在那里它被整合到核DNA中并表达， 最终导致转化的肿瘤生长 细胞 Ti质粒的大多数vir基因编码 参与T-DNA加工和转移的蛋白质 直到细菌接受酚类物质后 受伤的植物细胞产生的分子。 推定的 该信号的受体（VirA）已通过遗传学方法鉴定。 是指92 kDa的细胞质膜定位蛋白。 一 提出了苯酚/受体蛋白的模型 协会和描述的化学，生物化学和 分子遗传学方法的表征， 提供了互动。 这个简单的模型系统 应该可以提供对信号机制的深入了解， 酚类物质介导的转导。 %%% 有了这个生命过程化学奖，合成 化学系有机课程与细胞 细胞生物科学系生物化学专业 将支持大卫G.林恩的 美国芝加哥大学化学系教授。 安德鲁·N大学生物系的宾斯 宾夕法尼亚州。 研究的主要重点是研究 酚类化合物作为信号分子在真核生物和 和原核生物。 重要的激素和生长 动物界和植物界的因素都属于这一范围。 类化合物。 使用模式系统农杆菌 本项目将探讨农杆菌的作用机制， 酚类物质与特定受体相互作用， 细胞反应。 长期研究目标是 了解植物的发展。

This research will study the mechanism(s) whereby phenolics interact with specific receptors to elicit cellular responses. An important model system that cues on phenolics as signals is Agrobacterium tumefaciens, a pathogenic soil bacterium that causes tumors at wound sites of infected plants. The bacterium can transfer DNA (T-DNA) from its Ti plasmid into plant cells where it is integrated into the nuclear DNA and expressed, ultimately leading to the tumourous growth of the transformed cells. Most of the vir genes of the Ti plasmid that encode the proteins involved in the processing and transfer of the T-DNA are not expressed until the bacterium receives phenolic molecules produced by wounded plant cells. The putative receptor of this signal (VirA) has been identified by genetic means as a 92kDa cytoplasmic membrane localized protein. A model has been proposed for the phenol/receptor protein association and a description of chemical, biochemical and molecular genetic approaches for the characterization of the interaction have been provided. This simple model system should provide insight into the mechanisms of signal transduction mediated by phenolics. %%% With this Chemistry of Life Processes award, the Synthetic Organic Program of the Chemistry Division and the Cellular Biochemistry Program of the Division of Cellular Biosciences will support the research of Dr. David G. Lynn of the Department of Chemistry of the University of Chicago and Dr. Andrew N. Binns of the Department of Biology of the University of Pennsylvania. The major focus of the research is to study the role of phenolics as signal molecules in both eukaryotic and prokaryotic organisms. Important hormones and growth factors in both the Animal and Plant Kingdoms fall within this class of compounds. Using the model system Agrobacterium tumefaciens, the project will probe the mechanism(s) whereby the phenolics interact with specific receptors to elicit cellular responses. The long-range research goals are directed towards understanding plant development.