MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation Instrument

MRI：获得双尺寸排阻色谱-不对称流场流分馏仪器

• 批准号: 1126534
• 负责人: Judy Riffle
• 金额: $ 25.53万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-10-01 至 2015-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1126534&HistoricalAwards=false
• 关键词: MRI; Acquisition; Dual; Size; Exclusion

MRI Proposal DMR 1126534_McGrath TECHNICAL SUMMARY MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation (SEC-AFFFF) Instrument, James McGrath, Kevin Edgar, Richey Davis, Richard Turner and Judy Riffle, Depts of Chemistry, Wood Science and Forest Products, Chemical Engineering and the Macromolecules and Interfaces Institute, VA Tech, Blacksburg, VA A centralized, multi-user SEC-AFFFF facility is requested to characterize absolute molecular weights and sizes of a diverse array of homo- and copolymers and macromolecular colloidal assemblies. The sizes of macromolecules in solution are intrinsically related to their molecular parameters such as the type, amount and locations of ionic groups, copolymer microstructure, hydrophobic/hydrophilic nature, architectures, phase structures, tacticities, and intra- and inter-molecular interactions. In turn, the sizes control functions such as mechanical, thermal, and transport properties as well as the propensity for cooperative complexation to assemble into supramolecular constructs. Polymer research at VA Tech emphasizes the synthetic chemistry of macromolecules, particularly on charged and multi-phase structures, high and low molecular weight materials with extensive hydrogen bonding, and on molecules with varied architectures, and such systems present great challenges for accurate characterization of their sizes. Moreover, our growing emphasis on natural polymers including functionally-modified polysaccharides and highly-charged nucleic acids present unique difficulties in measurements and interpretation of molecular weight analyses. An analytical SEC-AFFFF facility with multiple integrated detectors to measure on-line refractive indices, UV absorbance, dilute solution viscosities and light scattering, to handle aqueous and organic solvents, and to operate over a wide range of molecular weights is requested to characterize these polymers and self-assembled structures. The SEC-AFFFF facility will allow measurement of absolute molecular weights, intrinsic viscosities, virial coefficients, degrees of branching, aggregation characteristics, complex sizes, chemical stability, and biodegradation for a vast collection of chemical compositions. SEC and AFFFF methods will be integrated into undergraduate and graduate coursework, academic year research and summer undergraduate programs. Our goal is to impact society through research in alternative energy solutions, water purification, gas separations, medical diagnostics and new molecular carriers for therapeutics.NON-TECHNICAL SUMMARY: MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation (SEC-AFFFF) Instrument, James McGrath, Kevin Edgar, Richey Davis, Richard Turner and Judy Riffle, Depts of Chemistry, Wood Science and Forest Products, Chemical Engineering and the Macromolecules and Interfaces Institute, VA Tech, Blacksburg, VA Molecular weights and sizes of macromolecules and nanoscale polymer aggregates represent one of the most critical parameters that define their properties and potential applications. The sizes control functions such as mechanical, thermal, and transport properties as well as the capacity to assemble into nanomaterial complexes. Polymer research at VA Tech emphasizes the design, synthesis and properties of multiphase and charged polymers with a wide range of molecular weights that present great challenges for accurate characterization of their sizes. The functionality and phase structures of these polymers lead to materials for alternative energy solutions, water purification, gas separations, medical diagnostics and new molecular carriers for therapeutics. This proposal requests a dual SEC-AFFFF for a centralized, multi-user facility. SEC is the premier characterization tool for determining the molecular weights of both synthetic and natural polymers. AFFFF is a relatively new analytical method that particularly enables separations of very high molecular weight polymers, charged polymers, micelles, polymer-drug complexes and nanoparticles. The facility will be integrated into coursework and research at both the undergraduate and graduate levels, and will also be available to regional institutions outside of VA Tech.

MRI方案DMR 1126534_McGrath技术概述获得双尺寸排除色谱-不对称流场流动分选（SEC-AFFFF）仪器，James McGrath, Kevin Edgar, Richey Davis， Richard Turner和Judy Riffle，化学系，木材科学和森林产品，化学工程和大分子和界面研究所，弗吉尼亚理工学院，布莱克斯堡，弗吉尼亚州要求多用户SEC-AFFFF设备表征各种同质聚合物和共聚物以及大分子胶体组合的绝对分子量和大小。溶液中大分子的大小与它们的分子参数有内在的关系，如离子基团的类型、数量和位置、共聚物的微观结构、疏水/亲水性、结构、相结构、策略以及分子内和分子间的相互作用。反过来，尺寸控制功能，如机械，热和运输性质，以及倾向于合作络合组装成超分子结构。VA Tech的聚合物研究强调大分子的合成化学，特别是带电和多相结构，具有广泛氢键的高分子量和低分子量材料，以及具有不同结构的分子，这些系统对其尺寸的准确表征提出了巨大的挑战。此外，我们越来越重视天然聚合物，包括功能修饰的多糖和高电荷核酸，这在分子量分析的测量和解释方面存在独特的困难。需要一个具有多个集成检测器的SEC-AFFFF分析设备来测量在线折射率，紫外线吸收，稀溶液粘度和光散射，处理水性和有机溶剂，并在大分子量范围内操作，以表征这些聚合物和自组装结构。SEC-AFFFF设备将允许测量绝对分子量、固有粘度、维里系数、分支度、聚集特性、复杂尺寸、化学稳定性和大量化学成分的生物降解。SEC和AFFFF方法将整合到本科和研究生课程、学年研究和夏季本科课程中。我们的目标是通过研究替代能源解决方案、水净化、气体分离、医疗诊断和新的治疗分子载体来影响社会。非技术总结：mri：获得双尺寸排除色谱-不对称流场流动分选（SEC-AFFFF）仪器，James McGrath, Kevin Edgar, Richey Davis， Richard Turner和Judy Riffle，化学系，木材科学和林产品，化学工程和大分子和界面研究所，VA Tech, Blacksburg，高分子和纳米级聚合物聚集体的分子量和大小是决定其性质和潜在应用的最关键参数之一。尺寸控制的功能，如机械，热和运输性质，以及组装成纳米材料复合物的能力。VA Tech的聚合物研究强调多相和带电聚合物的设计、合成和性能，这些聚合物具有广泛的分子量，对其尺寸的准确表征提出了巨大的挑战。这些聚合物的功能和相结构使其成为替代能源解决方案、水净化、气体分离、医疗诊断和治疗新分子载体的材料。该提案要求为一个集中的多用户设施设置双重SEC-AFFFF。SEC是确定合成和天然聚合物分子量的首要表征工具。AFFFF是一种相对较新的分析方法，特别适用于极高分子量聚合物、带电聚合物、胶束、聚合物-药物复合物和纳米颗粒的分离。该设施将被整合到本科和研究生阶段的课程和研究中，也将提供给弗吉尼亚理工学院以外的地区机构。