MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation Instrument

核磁共振提案DMR 1126534_McGrath核磁共振：收购双尺寸排除色谱-不对称流场流动分离(SEC-AFFFF)仪器，詹姆斯·麦格拉思，凯文·埃德加，里奇·戴维斯，理查德·特纳和朱迪·里弗尔，化学，木材科学和森林产品，化学工程，高分子和界面研究所，VA理工学院，布莱克斯堡，弗吉尼亚州，一个集中的，多用户的SEC-AFFFF设备，以表征不同阵列的同质和共聚物以及大分子胶体组件的绝对分子量和大小。大分子在溶液中的大小与其分子参数如离子基团的类型、数量和位置、共聚物的微观结构、疏水/亲水性质、结构、相结构、触感以及分子内和分子间的相互作用等有着内在的联系。反过来，大小控制着诸如机械、热和传输性质等功能，以及协同络合组装成超分子结构的倾向。弗吉尼亚理工学院的聚合物研究侧重于大分子的合成化学，特别是带电和多相结构、具有广泛氢键的高和低分子材料以及具有不同结构的分子，这些系统对准确表征其大小提出了巨大的挑战。此外，我们越来越重视天然聚合物，包括功能修饰的多糖和高电荷核酸，这给分子量分析的测量和解释带来了独特的困难。为了表征这些聚合物和自组装结构，需要一台带有多个集成检测器的分析SEC-AFFFF设备，以在线测量折射率、UV吸光度、稀溶液粘度和光散射，处理水和有机溶剂，并在广泛的分子量范围内操作。SEC-AFFFF设施将允许测量大量化学成分的绝对分子量、特性粘度、维里系数、支化度、聚集特征、复杂尺寸、化学稳定性和生物降解性。SEC和AFFFF方法将被整合到本科生和研究生课程、学年研究和夏季本科生计划中。我们的目标是通过对替代能源解决方案、水净化、气体分离、医疗诊断和治疗用新型分子载体的研究来影响社会。非技术摘要：MRI：收购双尺寸排除色谱-不对称流场分离(SEC-AFFFF)仪器，James McGrath，Kevin Edgar，Riceh Davis，Richard Turner和Judy Riffle，化学、木材科学和森林产品部，化学工程和大分子和界面研究所，VA理工学院，Blacksburg，VA大分子和大分子和纳米级聚合物聚集体的分子量和大小代表着定义其特性和潜在应用的最关键的参数之一。尺寸控制诸如机械、热和传输特性等功能，以及组装成纳米材料复合体的能力。弗吉尼亚理工学院的聚合物研究侧重于多相和带电聚合物的设计、合成和性能，这些聚合物的相对分子质量范围很大，这对准确表征其尺寸提出了巨大挑战。这些聚合物的功能和相结构导致了用于替代能源解决方案、水净化、气体分离、医疗诊断和治疗的新型分子载体的材料。该提案要求为集中式多用户设施配备双SEC-AFFFF。SEC是测定合成和天然聚合物分子量的首选表征工具。AFFFF是一种相对较新的分析方法，尤其能够分离超高分子量聚合物、带电聚合物、胶束、聚合物-药物络合物和纳米颗粒。该设施将整合到本科生和研究生的课程和研究中，并将向退伍军人技术学院以外的地区机构开放。

MRI Proposal DMR 1126534_McGrath TECHNICAL SUMMARY MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation (SEC-AFFFF) Instrument, James McGrath, Kevin Edgar, Richey Davis, Richard Turner and Judy Riffle, Depts of Chemistry, Wood Science and Forest Products, Chemical Engineering and the Macromolecules and Interfaces Institute, VA Tech, Blacksburg, VA A centralized, multi-user SEC-AFFFF facility is requested to characterize absolute molecular weights and sizes of a diverse array of homo- and copolymers and macromolecular colloidal assemblies. The sizes of macromolecules in solution are intrinsically related to their molecular parameters such as the type, amount and locations of ionic groups, copolymer microstructure, hydrophobic/hydrophilic nature, architectures, phase structures, tacticities, and intra- and inter-molecular interactions. In turn, the sizes control functions such as mechanical, thermal, and transport properties as well as the propensity for cooperative complexation to assemble into supramolecular constructs. Polymer research at VA Tech emphasizes the synthetic chemistry of macromolecules, particularly on charged and multi-phase structures, high and low molecular weight materials with extensive hydrogen bonding, and on molecules with varied architectures, and such systems present great challenges for accurate characterization of their sizes. Moreover, our growing emphasis on natural polymers including functionally-modified polysaccharides and highly-charged nucleic acids present unique difficulties in measurements and interpretation of molecular weight analyses. An analytical SEC-AFFFF facility with multiple integrated detectors to measure on-line refractive indices, UV absorbance, dilute solution viscosities and light scattering, to handle aqueous and organic solvents, and to operate over a wide range of molecular weights is requested to characterize these polymers and self-assembled structures. The SEC-AFFFF facility will allow measurement of absolute molecular weights, intrinsic viscosities, virial coefficients, degrees of branching, aggregation characteristics, complex sizes, chemical stability, and biodegradation for a vast collection of chemical compositions. SEC and AFFFF methods will be integrated into undergraduate and graduate coursework, academic year research and summer undergraduate programs. Our goal is to impact society through research in alternative energy solutions, water purification, gas separations, medical diagnostics and new molecular carriers for therapeutics.NON-TECHNICAL SUMMARY: MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation (SEC-AFFFF) Instrument, James McGrath, Kevin Edgar, Richey Davis, Richard Turner and Judy Riffle, Depts of Chemistry, Wood Science and Forest Products, Chemical Engineering and the Macromolecules and Interfaces Institute, VA Tech, Blacksburg, VA Molecular weights and sizes of macromolecules and nanoscale polymer aggregates represent one of the most critical parameters that define their properties and potential applications. The sizes control functions such as mechanical, thermal, and transport properties as well as the capacity to assemble into nanomaterial complexes. Polymer research at VA Tech emphasizes the design, synthesis and properties of multiphase and charged polymers with a wide range of molecular weights that present great challenges for accurate characterization of their sizes. The functionality and phase structures of these polymers lead to materials for alternative energy solutions, water purification, gas separations, medical diagnostics and new molecular carriers for therapeutics. This proposal requests a dual SEC-AFFFF for a centralized, multi-user facility. SEC is the premier characterization tool for determining the molecular weights of both synthetic and natural polymers. AFFFF is a relatively new analytical method that particularly enables separations of very high molecular weight polymers, charged polymers, micelles, polymer-drug complexes and nanoparticles. The facility will be integrated into coursework and research at both the undergraduate and graduate levels, and will also be available to regional institutions outside of VA Tech.