MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation Instrument

MRI提案DMR 1126534_McGrath技术总结MRI：双排阻色谱-不对称流场流动分级的获得（SEC-AFFFF）仪器，James麦格拉思，Kevin埃德加，里奇戴维斯，理查德特纳和朱迪里弗尔，化学部，木材科学和森林产品，化学工程和高分子和界面研究所，弗吉尼亚州技术，布莱克斯堡，弗吉尼亚州A集中，多用户SEC-AFFFF设备被要求表征绝对分子量和大小的各种各样的均聚物和共聚物和大分子胶体组装。溶液中大分子的大小与其分子参数如离子基团的类型、数量和位置、共聚物微观结构、疏水/亲水性质、结构、相结构、构象以及分子内和分子间相互作用等内在相关。反过来，尺寸控制功能，如机械，热，和运输性能，以及倾向于合作络合组装成超分子结构。VA Tech的聚合物研究强调大分子的合成化学，特别是带电和多相结构，具有广泛氢键的高分子量和低分子量材料，以及具有不同结构的分子，这些系统对精确表征其尺寸提出了巨大挑战。此外，我们越来越重视天然聚合物，包括功能修饰的多糖和高电荷核酸，这给分子量分析的测量和解释带来了独特的困难。一个分析SEC-AFFFF设施与多个集成的检测器，以测量在线折射率，紫外吸收，稀溶液粘度和光散射，处理水和有机溶剂，并在宽范围的分子量范围内操作，要求这些聚合物和自组装结构的特征。SEC-AFFFF设施将允许测量绝对分子量、特性粘度、维里系数、支化度、聚集特性、复杂尺寸、化学稳定性和大量化学成分的生物降解。SEC和AFFFF方法将被整合到本科和研究生课程，学年研究和夏季本科课程中。我们的目标是通过研究替代能源解决方案、水净化、气体分离、医疗诊断和治疗用新分子载体来影响社会。双排阻色谱-不对称流场流动分级的获得（SEC-AFFFF）仪器，James麦格拉思，Kevin埃德加，里奇戴维斯，理查德特纳和朱迪里弗尔，化学，木材科学和森林产品部，化学工程和高分子与界面研究所，VA Tech，Blacksburg，VA大分子和纳米级聚合物聚集体的分子量和尺寸代表了定义其性质和潜在应用的最关键参数之一。尺寸控制功能，如机械，热和运输性能以及组装成纳米材料复合物的能力。VA Tech的聚合物研究强调多相和带电聚合物的设计，合成和性能，这些聚合物具有广泛的分子量，对精确表征其尺寸提出了巨大的挑战。这些聚合物的功能和相结构导致替代能源解决方案，水净化，气体分离，医疗诊断和治疗新分子载体的材料。该提案要求为集中式多用户设施提供双SEC-AFFF。SEC是测定合成和天然聚合物分子量的首要表征工具。AFFFF是一种相对较新的分析方法，特别能够分离非常高分子量的聚合物、带电聚合物、胶束、聚合物-药物复合物和纳米颗粒。该设施将被整合到课程和研究在本科和研究生水平，也将提供给VA技术以外的区域机构。

MRI Proposal DMR 1126534_McGrath TECHNICAL SUMMARY MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation (SEC-AFFFF) Instrument, James McGrath, Kevin Edgar, Richey Davis, Richard Turner and Judy Riffle, Depts of Chemistry, Wood Science and Forest Products, Chemical Engineering and the Macromolecules and Interfaces Institute, VA Tech, Blacksburg, VA A centralized, multi-user SEC-AFFFF facility is requested to characterize absolute molecular weights and sizes of a diverse array of homo- and copolymers and macromolecular colloidal assemblies. The sizes of macromolecules in solution are intrinsically related to their molecular parameters such as the type, amount and locations of ionic groups, copolymer microstructure, hydrophobic/hydrophilic nature, architectures, phase structures, tacticities, and intra- and inter-molecular interactions. In turn, the sizes control functions such as mechanical, thermal, and transport properties as well as the propensity for cooperative complexation to assemble into supramolecular constructs. Polymer research at VA Tech emphasizes the synthetic chemistry of macromolecules, particularly on charged and multi-phase structures, high and low molecular weight materials with extensive hydrogen bonding, and on molecules with varied architectures, and such systems present great challenges for accurate characterization of their sizes. Moreover, our growing emphasis on natural polymers including functionally-modified polysaccharides and highly-charged nucleic acids present unique difficulties in measurements and interpretation of molecular weight analyses. An analytical SEC-AFFFF facility with multiple integrated detectors to measure on-line refractive indices, UV absorbance, dilute solution viscosities and light scattering, to handle aqueous and organic solvents, and to operate over a wide range of molecular weights is requested to characterize these polymers and self-assembled structures. The SEC-AFFFF facility will allow measurement of absolute molecular weights, intrinsic viscosities, virial coefficients, degrees of branching, aggregation characteristics, complex sizes, chemical stability, and biodegradation for a vast collection of chemical compositions. SEC and AFFFF methods will be integrated into undergraduate and graduate coursework, academic year research and summer undergraduate programs. Our goal is to impact society through research in alternative energy solutions, water purification, gas separations, medical diagnostics and new molecular carriers for therapeutics.NON-TECHNICAL SUMMARY: MRI: Acquisition of a Dual Size Exclusion Chromatography-Asymmetric Flow Field Flow Fractionation (SEC-AFFFF) Instrument, James McGrath, Kevin Edgar, Richey Davis, Richard Turner and Judy Riffle, Depts of Chemistry, Wood Science and Forest Products, Chemical Engineering and the Macromolecules and Interfaces Institute, VA Tech, Blacksburg, VA Molecular weights and sizes of macromolecules and nanoscale polymer aggregates represent one of the most critical parameters that define their properties and potential applications. The sizes control functions such as mechanical, thermal, and transport properties as well as the capacity to assemble into nanomaterial complexes. Polymer research at VA Tech emphasizes the design, synthesis and properties of multiphase and charged polymers with a wide range of molecular weights that present great challenges for accurate characterization of their sizes. The functionality and phase structures of these polymers lead to materials for alternative energy solutions, water purification, gas separations, medical diagnostics and new molecular carriers for therapeutics. This proposal requests a dual SEC-AFFFF for a centralized, multi-user facility. SEC is the premier characterization tool for determining the molecular weights of both synthetic and natural polymers. AFFFF is a relatively new analytical method that particularly enables separations of very high molecular weight polymers, charged polymers, micelles, polymer-drug complexes and nanoparticles. The facility will be integrated into coursework and research at both the undergraduate and graduate levels, and will also be available to regional institutions outside of VA Tech.