Kinetics of Growth And Product Formation In Hairy Root Cultures

主要研究人员计划研究万寿菊（万寿菊）快速生长的毛状根培养物的生长动力学和噻吩的产生。噻吩是聚乙炔的硫衍生物，主要存在于这些植物的根中。最近的研究结果表明，它们可能在保护植物免受植物病原真菌、昆虫和线虫的侵害方面发挥作用。因此，噻吩可能是一种对环境安全的传统农药替代品。这个项目的目标是：-建立并求解黄芪毛状根无性系生物量生长和噻吩积累的数学模型万寿菊的物种。-根据摇瓶中培养的诱导和非诱导万寿菊毛状根无性系的生长数据确定这些模型中的动力学参数。-通过与生物反应器生长根的生长数据进行比较，测试各种模型的有效性，并使用最佳数学模型优化生物反应器性能。

The Principal Investigators (PIs) plan to investigate the kinetics of growth and thiophene production from fast-growing hairy root cultures of the Tagetes species (marigolds). Thiophenes are sulfur derivatives of polyacetylenes, and are found mainly in the roots of these plants. Recent findings suggest that they may play a role in protecting the plant from phytopathogenic fungi, insects, and nematodes. Thiophenes may, therefore, provide an environmentally safe alternative to conventional pesticides. The objectives of this project are to: - Formulate and solve mathematical models of biomass growth and thiophene accumulation in hairy root clones of the Tagetes species. - Determine kinetic parameters in these models from growth data of elicited and non-elicited Tagetes hairy root clones cultured in shake flasks. - Test the validity of various models by comparison with growth data from bioreactor grown roots and to optimize the bioreactor performance using the best mathematical models.