Root Cultures for the Production of Biologically Active Plant Secondary Metabolites

用于生产生物活性植物次生代谢物的根培养

• 批准号: 8713078
• 负责人: Martin Hjortso
• 金额: $ 68.08万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8713078&HistoricalAwards=false
• 关键词: Root; Cultures; Production; Biologically; Active

Secondary metabolites (products) from plant roots are under- utilized and under-explored sources of pharmaceuticals and agrichemicals. Roots of the Asteraceae (Sunflower family) accumulate a wide range of valuable natural products including fungicidal, anti-bacterial, antiviral and nematicidal polyacetylenes. The mechanisms of synthesis and accumulation for most of these compounds are poorly understood. The PIs have recently developed fast-growing root cultures from various Solanceae and Asteraceae species which have been transformed into "hairy roots" following infection with the bacterium Agrobacterium rhizogenes. These "hairy root" cultures grow at rates faster than normal roots and comparable to those of undifferentiated cell suspensions; at the same time, they continue producing the secondary metabolites characteristic of roots. The PIs suggest that this experimental system is well suited for development into a large-scale production process. Large bioreactors will be built for the growth of root clones in batch, semi-continuous or continuous culture. This grant application is an excellent example of the type of crossdisciplinary proposal we wish to see in the Engineering Directorate's Biotechnology Program. It was rated excellent by the crossdisciplinary Panel reviewing this proposal and the Principal Investigators are considered well qualified to carry out the proposed research. I recommend funding of this proposal for three years in accordance with the revised budget.

植物根的次级代谢产物（产品）在- 已利用和未充分开发的药品来源， 农药 菊科（向日葵科）的根 积累了广泛的有价值的天然产品，包括 杀真菌、抗菌、抗病毒和杀线虫的聚乙炔。 其中大部分的合成和积累机制 对化合物了解甚少。 PI最近开发了快速生长的根文化， 各种茄科和菊科物种， 转化成“毛状根”后，感染 细菌发根农杆菌。 这些“毛状根”培养物 比普通根的生长速度快， 未分化的细胞悬浮液;同时，它们继续 产生根特有的次生代谢物。 法律与正义党 表明该实验系统非常适合于开发 进入大规模生产过程。 将建造大型生物反应器 用于批量、半连续或连续的根克隆生长 文化 这份补助金申请是一个很好的例子， 我们希望在工程中看到的跨学科建议 董事会的生物技术计划。 它被评为优秀， 审查这一建议的跨学科小组和 主要研究者被认为有资格进行 提出的研究。 我建议为这项提案提供资金 根据修订后的预算，三年。