Growth and Protein Expression of Eukaryotic Cells

该提案旨在评估细胞生长的动力学，以及在单个真核细胞中使用理论和实验工具合成大分子，这些工具在之前的奖项中已经开发出来。这些工具将继续发展，并将允许在单细胞水平上绘制真核细胞群体在各种环境条件下的动态模式。这项研究的工程效益将是动力学模型比传统的非隔离模型具有更好的预测价值。

This proposal seeks to evaluate the kinetics of cell growth, and the synthesis of macromolecules in single eukaryotic cells with theoretical and experimental tools that have been developed during a previous award. These tools will continue to be developed, and will permit the mapping of the dynamic patterns of eukaryotic cell populations under a variety of environmental conditions at the single cell level. The engineering benefit of this study will be kinetic models that have a much better predictive value than conventional non-segregated models.