Development of On-line Flow Cytometry

本项目的目的是开发一个在线流式细胞仪系统。分选流式细胞仪将与生物反应器连接。该接口将允许对细胞进行自动取样和染色。在线流式细胞仪系统的主要应用将是监测单细胞水平的培养生理。具体而言，将进行实验以确定大肠杆菌的单细胞葡萄糖摄取率，酿酒葡萄球菌细胞进行有丝分裂的比例，并最终分离大肠杆菌转运突变体。

The objective of this project is to develop an on-line flow cytometry system. A sorting flow cytometer will be interfaced to a bioreactor. The interface will allow automatic sampling and staining of the cells. The primary application of the on-line flow cytometry system will be on monitoring culture physiology at the single-cell level. Specifically, experiments will be carried out to determine single cell glucose uptake rates for E. coli, the fraction of S. cerevisae cells undergoing mitosis and, finally, for the isolation of E. coli transport mutants.