Growth and Protein Expression of Eukaryotic Cells

9414385 Srienc本项目是以酿酒酵母和小鼠杂交瘤为真核模型系统，在单细胞水平上研究真核细胞种群的动态。流式细胞术技术已经被开发出来，它允许通过实验确定平衡生长条件下的速率、转变和分配函数。这些基本的生理功能，与种群平衡方程相结合，严格描述了细胞种群的生长动力学。这种方法现在将扩展到评估这些功能如何依赖于环境生长参数，如特定营养物质的浓度，目的是更好地了解生物反应器中的瞬时生长情况。此外，该项目将侧重于实验确定单细胞生长率与单细胞营养素消耗和产品形成之间的关系。***

9414385 Srienc This project is on research to study the dynamics of eukaryotic cell populations at the single cell level using Saccharomyces cerevisiae and murine hybridomas as eukaryotic model systems. Flow cytometry techniques have been developed which permit experimental determination of the rate, transition, and partitioning functions for balanced growth conditions. These fundamental physiological functions, in combination with population balance equations, rigorously describe the growth dynamics of cell populations. This approach will now be extended to evaluate how these functions depend on environmental growth parameters, such as concentrations of specific nutrients, with the aim to better understand transient growth situations in a bioreactor. Also, this project will focus on experimental determination of the relationship between single-cell growth rates and single-cell rates of nutrient consumption and product formation. ***