Investigation of gene and/or protein expression associated with recovery and/or regeneration of skeletal muscle from growth inhibition or damage

研究与骨骼肌从生长抑制或损伤中恢复和/或再生相关的基因和/或蛋白质表达

• 批准号: 15200049
• 负责人: OHIRA Yoshinobu
• 金额: $ 32.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15200049/
• 关键词: Skeletal muscle; Immune function; Proteomics; Regeneration; プロテオーム; 障害; 老化; 回復; タンパク質; 遺伝子; 衛星細胞

It has been well-known that the decreases of morphological and/or functional properties of skeletal muscles, caused by growth retardation, injury, long-term bedrest, spaceflight, or aging, inhibit the quality of life in daily life. Therefore, it is essential to develop the suitable methodology for the promotion of recovery and/or regeneration of muscles with inhibited properties or injury. Role(s) of interleukin-6 (IL-6), macrophage, satellite cell, and mechanical and/or metabolic stimuli in the regulation of muscular properties in response to damage or regeneration were studied. As the results, it was suggested that satellite cells play one of the key roles in the plasticity of muscle. The number and activity of satellite cells were decreased in muscle with atrophy or damage. On the contrary, data indicated that the recoveries of these parameters are essential for the regeneration of muscle. It was also suggested that the number and activity of satellite cells are regulated by mechanical stimuli, heat stress, IL-6, and macrophage. Further, it was indicated that the responses of gene and protein expression in rat soleus muscle to growth retardation, caused by gravitational unloading, and recovery in 1-G environment did not always coincide.

众所周知，由于生长迟缓、损伤、长期卧床、航天或衰老引起的骨骼肌形态和/或功能特性的降低抑制了日常生活中的生活质量。因此，必须开发合适的方法来促进具有抑制性质或损伤的肌肉的恢复和/或再生。研究了白细胞介素-6（IL-6）、巨噬细胞、卫星细胞以及机械和/或代谢刺激在肌肉特性响应损伤或再生的调节中的作用。结果表明，卫星细胞在肌肉的可塑性中起着重要作用。萎缩或损伤的肌肉中卫星细胞的数量和活性降低。相反，数据表明，这些参数的恢复是必不可少的肌肉再生。卫星细胞的数量和活性受机械刺激、热应激、IL-6和巨噬细胞的调节。此外，这表明，基因和蛋白质表达的反应，大鼠比目鱼肌生长迟缓，造成的重力卸载，并在1-G环境中的恢复并不总是一致的。

项目成果

Role of gravity in mammalian development : effects of hypergravity and/or microgravity on the development of skeletal muscles.

重力在哺乳动物发育中的作用：超重力和/或微重力对骨骼肌发育的影响。

• 发表时间: 2004
• 期刊: Biol. Sci. Space 18
• 作者: Ohira;Y.;F.Kawano;K.Goto;X.D.Wang;Y.Takeno;A.Ishihara
• 通讯作者: A.Ishihara

Effects of mechanical and neural stimuli on the properties of soleus muscle fibers in mdx mice.

机械和神经刺激对 mdx 小鼠比目鱼肌纤维特性的影响。

• 期刊: Jpn. J. Aerosp. Environ. Med. (in press)
• 作者: Lan;Y.B.;M.Terada;Y.Higo;F.Kawano;Y.Matsuoka;X.D.Wang;N.Nakai;Y.Ohira
• 通讯作者: Y.Ohira

Profiles of connectin (titin) in atrophied soleus muscle induced by unloading of rats

• DOI: 10.1152/japplphysiol.00408.2002
• 发表时间: 2003-03-01
• 期刊: JOURNAL OF APPLIED PHYSIOLOGY
• 影响因子: 3.3
• 作者: Goto, K;Okuyama, R;Yoshioka, T
• 通讯作者: Yoshioka, T

現代 老年精神医療。 第一部.老化過程と高齢者4.身体の老化 身体能力の老化

现代老年精神病学。

• 作者: 後藤 勝正;大平 充宣
• 通讯作者: 大平 充宣

Responses of adductor longus muscle to hindlimb suspension in Wistar Hannover rats.

Wistar Hannover 大鼠内收长肌对后肢悬挂的反应。

• 发表时间: 2006
• 期刊: J. Gravit. Physiol. 13
• 作者: Ohira;T.;S.Morikuni;X.D.Wang;M.Terada;F.Kawano;Y.Matsuoka;Y.Higo;Y.B.Lan;T.Seki;Y.Ohira
• 通讯作者: Y.Ohira