RUI: Acquisition of an Image Analysis System for Microscopy

这项提案要求为显微镜和图像分析系统提供资金，该系统将允许分析来自许多不同生物系统的各种图像。显微镜是研究生物体的一种古老但非常强大的仪器。很少有其他生物工具允许在水环境中研究亚细胞结构、整个细胞，甚至是小型生物，通常是在活的状态下。新的细胞结构和功能探针，如荧光标记的抗体、DNA探针以及钙和pH依赖的荧光团，为探索细胞提供了更强大的工具。计算机与显微镜的结合已被证明是从图像中获取和分析数据的一种特别强大的手段。该建议中描述的研究项目包括各种生物系统，包括植物与病毒的相互作用、蓝藻的发育、神经再生、光学通路、水蚤的发育和黄蜂的进化。这些项目都需要一个系统来获取、增强和分析图像。

This proposal requests funds for a microscope and image analysis system that will allow analysis of a wide variety of images from many different biological system. The microscope is an old but very powerful instrument for the study of living organisms. Few other biological tools allow subcellular structures, whole cells, or even small organisms to be studied in an aqueous environment, frequently in a living state. New probes for cells structure and function such as fluorescently labelled antibodies, DNA probes, and calcium and pH dependent fluorophores provide even more powerful implements for exploring cells. The computer in combination with the microscope has proven to be a particularly powerful means for acquiring and analyzing data from images. The research projects described in this proposal encompass a variety of biological systems including plant-virus interactions, cyanobacterial development, nerve regeneration, optical pathways, leech development, and wasp evolution. These projects share a need for a system to acquire, enhance, and analyze images.