Domains of Gene Activity in Developing Embryos of Brassica napus

甘蓝型油菜胚胎发育中的基因活性域

• 批准号: 9105527
• 负责人: Donna Fernandez
• 金额: $ 27.6万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105527&HistoricalAwards=false
• 关键词: Domains; Gene; Activity; Developing; Embryos

The goal of the proposed research is to further our understanding of the role spatial information plays in regulating embryo development and the transition to germination in higher plants. The approach will be to use molecular probes and in situ localization techniques to look at the cellular basis for patterns of gene expression in Brassica napus embryos, and to examine how these patterns change over the course of development. An important consequence of this research will be there identification of clones that can be used to look at the molecular anatomy of the processes that appear to involve pattern formation, particularly the determination of the shoot apical meristem during early embryogenesis and the determination of organ identity at the shoot apex during precocious germination. These clones can also be used, in combination with genetic analysis of developmental mutants in Arabdopsis to dissect the mechanisms of pattern formation in plant embryos.

拟议研究的目标是进一步促进我们的 了解空间信息在调节 胚胎发育和向萌发的过渡 植物该方法将使用分子探针和原位 定位技术来观察细胞基础， 甘蓝型油菜胚中的基因表达模式， 研究这些模式在发展过程中如何变化。 这项研究的一个重要结果将在那里 鉴定可用于观察 分子解剖学的过程，似乎涉及模式 形成，特别是茎尖的确定 分生组织在早期胚胎发生和测定 在早熟萌发期间茎尖的器官特性。 这些克隆也可以与遗传学结合使用。 分析发育突变体在阿拉伯，解剖 植物胚胎中模式形成的机制。