Microanalytical Methods in Neurochemistry

对尤因的研究的重新支持， 神经递质和相关的分析化学来自 化学分析与表面化学专业 行为和心理学中的突触机制计划 神经科学。 研究将包括组装 电喷雾飞行时间质谱仪，将 用于识别和定量脂质和磷脂 完整的细胞膜 此外，来自单个细胞的神经肽 将识别出传递器囊泡。 质谱 测量将补充微电化学检测， 用于表征神经传递的神经递质 和转导。 %%% 虽然已知有多种小分子可以传播 信号从一个神经细胞传递到下一个， 分子对于理解神经传递也很重要 和神经细胞功能。 其中有脂肪物质 （脂质和磷脂）在细胞膜，其组成 它沿着细胞膜并随着细胞的状态而变化。 质谱法的使用将允许鉴定和 这些分子中的少量分子的结构解析来自 单细胞 此外，肽（小链或低聚物）也可用于治疗癌症。 氨基酸），其传递或改变 神经元信号将被识别和测序。

Renewed support for Ewing's research in sensing of neurotransmitters and related analytical chemistry comes from the Analytical and Surface Chemistry Program in the Chemistry Division and the Synaptic Mechanisms Program in Behavioral and Neural Sciences. Research will include assembly of an electrospray time-of-flight mass spectrometer, which will be used to identify and quantify lipids and phospholipids from intact cell membranes. In addition, neuropeptides from single transmitter vesicles will be identified. Mass spectral measurements will complement microelectrochemical detection of neurotransmitters for characterization of neural transmission and transduction. %%% While a variety of small molecules are known to transmit signals from one nerve cell to the next, a variety of larger molecules are also important in understanding neurotransmission and nerve cell function. Among these are fatty substances (lipids and phospholipids) in cell membranes, whose composition varies along the cell membrane and with the state of the cell. The use of mass spectrometry will allow identification and structure elucidation of small numbers of these molecules from single cells. In addition, peptides (small chains or oligomers of amino acids) which transmit or modify transmission of neuronal signals will be identified and sequenced.