Microanalytical Methods in Neurochemistry

神经化学中的微量分析方法

• 批准号: 9407306
• 负责人: Andrew Ewing
• 金额: $ 40.46万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-15 至 1997-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9407306&HistoricalAwards=false
• 关键词: Microanalytical; Methods; Neurochemistry

This research project, supported in the Analytical and Surface Chemistry Program, concerns the combination of capillary electrophoresis in narrow-bore capillaries with sensitive and selective detection methods to address several unique experiments in cellular neuroscience. The three fundamental goals of this project are to develop ultra-low volume separation methods to dynamically and selectively follow chemical release from single nerve cells, growth cones, and synapses; to develop capillary electrophoresis-mass spectrometry to quantify phospholipids in membranes of whole cells, regional sections of the plasma membrane, and axonal growth cones in culture; and to develop methods to identify and quantify chemical messengers in single developing growth cones. These techniques will be brought to bear on two important areas of neurobiology, namely neural development through the study of growth cones and cell-to-cell communication through events at the membrane. The analytical methodology development to be pursued in this project, which builds upon results obtained by Professor Ewing and his students during the tenure of NSF grant CHE-9106832, is important to the achievement of dimensionally and molecularly selective neurochemical measurements at the single cell level. These general methodologies are also important in biotechnology and can be expected to contribute to the development of essential instrumentation for environmental studies

该研究项目由分析和表面化学项目支持，涉及窄孔毛细血管毛细管电泳与敏感和选择性检测方法的结合，以解决细胞神经科学中的几个独特实验。该项目的三个基本目标是开发超低体积分离方法，以动态和选择性地跟踪单个神经细胞，生长锥和突触的化学释放；建立毛细管电泳-质谱联用技术，定量培养中全细胞膜、质膜局部切片和轴突生长锥的磷脂含量；建立鉴定和定量单个生长锥中化学信使的方法。这些技术将用于神经生物学的两个重要领域，即通过生长锥研究的神经发育和通过膜上事件的细胞间通信。在这个项目中，分析方法的发展是建立在尤因教授和他的学生在NSF资助CHE-9106832任期内获得的结果之上的，这对于在单细胞水平上实现维度和分子选择性神经化学测量是很重要的。这些一般方法在生物技术方面也很重要，可望有助于发展环境研究的基本仪器