Continuous Electrophoretic Separations in Micron and Submicron Channels

微米和亚微米通道中的连续电泳分离

• 批准号: 9727555
• 负责人: Andrew Ewing
• 金额: $ 55万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9727555&HistoricalAwards=false
• 关键词: Continuous; Electrophoretic; Separations; Micron; Submicron

This renewal project, supported in the Analytical and Surface Chemistry Program, focusses on the development of new continuous electrophoretic separations in narrow channels coupled to capillary sample introduction. Professor Ewing and his students at Penn State University will pursue work to improve detection schemes for these separations using electrochemical arrays and post-separation derivatization for fluorescence detection. The goal of this work is to develop a fast continuous or sequential analysis protocol for very small samples taken from neuronal environments. Another focus of this work will be in using these methods to monitor enzymatic reactions in order to do drug screening. This project has both fundamental and applied goals that will advance the sensitivity and speed of small sample analyses. New developments in continuous electrophoretic separations of very small samples in narrow channels will be pursued in this project. Professor Ewing and his students at Penn State University will focus on substantial improvements in the detection limits for these separations using both electrochemical and fluorescence methods of analysis. An important aspect of this work will be enhancing the speed of these analyses using very small samples so that screening methods can be developed in fields such as neurochemistry and drug development. This project has both fundamental scientific components and important applied goals that will be of societal value.

该更新项目得到了分析和表面化学项目的支持，重点是在窄通道中开发新的连续电泳分离，并将其与毛细管样品引入相结合。 宾夕法尼亚州立大学的尤因教授和他的学生将继续努力，利用电化学阵列和分离后衍生荧光检测来改进这些分离的检测方案。 这项工作的目标是开发一个快速的连续或连续的分析协议，从神经元环境的非常小的样品。 这项工作的另一个重点将是使用这些方法来监测酶反应，以便进行药物筛选。 该项目具有基本和应用目标，将提高小样本分析的灵敏度和速度。 在窄通道中连续电泳分离非常小的样品的新发展将在这个项目中进行。 宾夕法尼亚州立大学的尤因教授和他的学生将重点关注使用电化学和荧光分析方法对这些分离的检测限的实质性改进。 这项工作的一个重要方面将是使用非常小的样本提高这些分析的速度，以便在神经化学和药物开发等领域开发筛选方法。 该项目既有基本的科学组成部分，也有重要的应用目标，将具有社会价值。