ROW/RPG: Molecular Approach to the Systematics of the Tubellaria (Platyhelminthes)

ROW/RPG：管虫系统学的分子方法（扁形动物）

• 批准号: 9108757
• 负责人: Marianne Litvaitis
• 金额: $ 1.8万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9108757&HistoricalAwards=false
• 关键词: ROW; RPG; Molecular; Approach; Systematics

This project investigates the evolutionary relationships among three groups of free-living flatworms. A highly conserved gene of the mitochondrial DNA will be amplified using a new technique called the polymerase chain reaction, or PCR. Once amplified, the gene will be sequenced. The amplified products will also be used to clone larger pieces of the mitochondrial DNA with which the order of other mitochondrial genes can be determined. Mitochondrial DNA gene order will be used to determine evolutionary relationships among the three groups of flatworms. This information will be a beginning for a long-term study on mitochondrial gene order and gene sequences of nuclear DNA of representatives of an entire family of flatworms. Many flatworms hold a key position in theories on the origin of multicellular animals. Therefore, a better understanding of relationships among flatworms will also have implications for a better understanding of the evolution of multicellular animals.

这个项目调查了三组自由生活的扁虫之间的进化关系。线粒体DNA中高度保守的基因将使用一种称为聚合酶链反应（PCR）的新技术进行扩增。一旦扩增，基因将被测序。扩增的产物也将用于克隆更大的线粒体DNA片段，从而确定其他线粒体基因的顺序。线粒体DNA基因顺序将用于确定三组扁虫之间的进化关系。这一信息将为对整个扁虫科代表的线粒体基因顺序和核DNA基因序列的长期研究奠定基础。许多扁虫在多细胞动物起源理论中占有重要地位。因此，更好地理解扁虫之间的关系也将对更好地理解多细胞动物的进化产生影响。