U.S.-France Cooperative Research: Three Dimensional Reconstruction of Immunocomplexes from Images of Single Particles

美法合作研究：从单颗粒图像三维重建免疫复合物

• 批准号: 9115355
• 负责人: Joachim Frank
• 金额: $ 1.25万
• 依托单位: Health Research Incorporated/New York State Department of Health
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1996-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9115355&HistoricalAwards=false
• 关键词: U.S.; France; Cooperative; Research; Three

This three-year award supports U.S.-France cooperative research in biophysics between Joachim Frank from the New York State Department of Health in Albany and Jean Lamy at the University of Tours. The objective of their research is to develop a methodology for localizing binding sites of antibodies on immunocomplexes. Specifically, they will work on mapping antigenic epitopes in A. Hemocyanin molecules. The methodology involves using electron microscopy and three dimensional reconstruction techniques. Dr. Frank and his group bring to this collaboration innovative technology for image reconstruction. The Albany Lab has developed a large specialized software system for three-dimension imaging of macromolecules and two-dimensional maps. This is complemented by the French laboratory's extensive experience in immunochemistry and protein chemistry of A. Hemocyanin. In addition the project will benefit from their previous collaboration on three-dimensional reconstruction. The use of this model system is of great interest to researchers in macromolecular chemistry. The proposed techniques will facilitate the mapping of binding sites of macromolecules, which cannot be crystallized and are too large for conventional x-ray crystallography. The knowledge gained from this project will be beneficial to researchers in biochemistry and molecular biology.

这个为期三年的奖项支持美国-法国合作研究 来自纽约州的约阿希姆·弗兰克 奥尔巴尼的卫生部和大学的让·拉米 关于图尔斯。 他们研究的目的是开发一种 用于定位抗体结合位点的方法 免疫复合物 具体来说，他们将致力于绘制地图 A.抗原表位血蓝蛋白分子。 的方法 包括使用电子显微镜和三维 重建技术。 弗兰克博士和他的团队 图像重建的协作创新技术。 的 奥尔巴尼实验室开发了一个大型的专业软件系统， 大分子的三维成像和二维成像 地图 这与法国实验室广泛的 免疫化学和蛋白质化学的经验。 血蓝蛋白 此外，该项目还将受益于 以前在三维重建方面的合作。 该模型系统的使用引起了研究人员的极大兴趣， 高分子化学 所提出的技术将有助于 大分子的结合位点的映射，这不能被 结晶的并且对于常规X射线来说太大 结晶学 从这个项目中获得的知识将是 对生物化学和分子生物学的研究人员有益。