Acquisition of a 200 kV Field Emission Gun Transmission Electron Microscope for Macromolecular Imaging

这项申请要求资金用于生物分子和大分子组件成像的中压冷冻电子显微镜。与其他现有显微镜相比，该仪器的亮度增加了500-1000倍，可以在高相干模式下工作，几乎消除了高分辨率下对比度传递函数的衰减，并大大提高了图像的分辨率。该显微镜将用于研究原核和真核核糖体的结构；线粒体外膜孔；骨骼快速抽动钙释放通道；以及细胞质动力蛋白运动。对于所有这些项目，这台显微镜将产生显著改善的图像，提供这些生物系统结构的更详细的视觉。

This application requests funds for an intermediate voltage cryo- electron microscope for the imaging of biological molecules and macromolecular assemblies. This instrument, with its 500-1000-fold increased brightness over other available microscopes enables operation in a high-coherence mode that virtually eliminates the damping of the contrast transfer function at high resolutions, and greatly enhances the resolution to which images can be made. The microscope will be used for studies that include the structure of prokaryotic and eukaryotic ribosomes; mitochondrial outer membrane pore; skeletal fast twitch calcium release channel; and cytoplasmic dynein motion. For all these projects this microscope will produce significantly improved images, providing a much more highly detailed vision of the structures of these biological systems.