Acquisition of a 200 kV Field Emission Gun Transmission Electron Microscope for Macromolecular Imaging

购买用于高分子成像的 200 kV 场发射枪透射电子显微镜

• 批准号: 9871347
• 负责人: Joachim Frank
• 金额: $ 76.57万
• 依托单位: Health Research Incorporated/New York State Department of Health
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9871347&HistoricalAwards=false
• 关键词: Acquisition; 200; kV; Field; Emission

This application requests funds for an intermediate voltage cryo- electron microscope for the imaging of biological molecules and macromolecular assemblies. This instrument, with its 500-1000-fold increased brightness over other available microscopes enables operation in a high-coherence mode that virtually eliminates the damping of the contrast transfer function at high resolutions, and greatly enhances the resolution to which images can be made. The microscope will be used for studies that include the structure of prokaryotic and eukaryotic ribosomes; mitochondrial outer membrane pore; skeletal fast twitch calcium release channel; and cytoplasmic dynein motion. For all these projects this microscope will produce significantly improved images, providing a much more highly detailed vision of the structures of these biological systems.

该申请要求为生物分子和大分子组装体成像的中压低温电子显微镜提供资金。该仪器的亮度比其他可用显微镜增加了500-1000倍，能够在高相干模式下操作，几乎消除了高分辨率下对比度传递函数的阻尼，并大大提高了图像的分辨率。该显微镜将用于研究，包括原核和真核核糖体的结构;线粒体外膜孔;骨骼肌快收缩钙释放通道;和细胞质动力蛋白运动。对于所有这些项目，该显微镜将产生显着改善的图像，提供这些生物系统的结构的更详细的视觉。