Functional Studies of Chicken Histocompatibility Antigens Bearing Ig V-like Domains

带有 Ig V 样结构域的鸡组织相容性抗原的功能研究

• 批准号: 9118199
• 负责人: Marcia Miller
• 金额: --
• 依托单位: Beckman Research Institute City of Hope
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-15 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118199&HistoricalAwards=false
• 关键词: Functional; Studies; Chicken; Histocompatibility; Antigens

Significant progress was made recently in defining the structure of the highly polymorphic B-G antigens encoded within the major histocompatibility complex of the chicken. It has been determined that B-G antigens are a family of molecules, divergent members of which are expressed on cells in several different tissues in addition to erythrocytes, including immune system cells. Determination of the sequences of some of the B-G family members has revealed the structural basis of B-G polymorphism, including the presence of hypervariable sequence diversity within an immunoglobulin v-like domain. B-G cytoplasmic tails are made up of heptad repeats of diverse sequence. B-G antigens have been found on the epithelia in the gut, cells in the liver, thrombocytes (highly phagocytic cells in birds), some lymphocytes, and cells within the stroma of the bursa and thumus. The objective of this project is to attempt to define the function (or functions) of the B-G antigens in light of the newly recognized sites where B-G molecules are now known to be expressed. The B-G antigens have long been known as blood group antigens of chickens. Until recently, the B-G antigens were thought to be expressed exclusively on red blood cells. They are now known to be expressed in various cell types and tissues throughout the chick, at various stages of development. Furthermore, they have been shown through DNA sequencing work to have structural resemblance to immunoglobulin genes. These new observations have led to the hypothesis that the B-G antigens may play some role in the chicken immune system. The goal of this study is to explore this hypothesis. The results of this study have the potential to shed new light on how the immune system of the chicken operates.

最近，在确定鸡主要组织相容性复合体中编码的高度多态性 B-G 抗原的结构方面取得了重大进展。已确定B-G抗原是一个分子家族，其不同成员在除红细胞之外的几种不同组织的细胞上表达，包括免疫系统细胞。一些 B-G 家族成员的序列测定揭示了 B-G 多态性的结构基础，包括免疫球蛋白 V 样结构域内存在高变序列多样性。 B-G 细胞质尾由不同序列的七肽重复组成。 B-G 抗原已在肠道上皮、肝脏细胞、血小板（鸟类中的高度吞噬细胞）、一些淋巴细胞以及法氏囊和拇指基质内的细胞中发现。该项目的目的是尝试根据目前已知 B-G 分子表达的新识别位点来定义 B-G 抗原的功能。 B-G抗原长期以来被称为鸡的血型抗原。直到最近，B-G 抗原还被认为只在红细胞上表达。现在已知它们在雏鸡各个发育阶段的各种细胞类型和组织中表达。此外，DNA测序工作表明它们与免疫球蛋白基因具有结构相似性。这些新的观察结果引发了这样的假设：B-G 抗原可能在鸡的免疫系统中发挥一定作用。本研究的目的是探索这一假设。这项研究的结果有可能为鸡的免疫系统如何运作提供新的线索。