Molecular analysis of plant-parasitic nematodes during their interactions with wild-type and mutant Arabidopsis plants

植物寄生线虫与野生型和突变拟南芥植物相互作用过程中的分子分析

• 批准号: 211111786
• 负责人: Dr. Cynthia Gleason
• 金额: --
• 依托单位: Abteilung Molekularbiologie und Physiologie der Pflanze
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/211111786?language=en
• 关键词: Molecular; analysis; plant; parasitic; nematodes

Plant-parasitic nematodes are a huge agricultural problem on many of the world’s main food crops. One of the most damaging of the plant-parasitic nematodes is the root-knot nematode. Root-knot nematodes are a serious agricultural threat due to their large host range and lack of natural plant resistance. During the susceptible interaction, root-knot nematodes invade host roots and choose plant cells to convert into metabolically-active feeding sites. The root-knot nematode’s manipulation of the plant cell, and in particular how the nematode is able to regulate host plant pathways, is not well-understood. The aim of this proposal is to further explore plant/root-knot nematode interactions. We want to identify and characterize nematode genes that are critical to successful parasitism as these would be excellent targets for nematode control. To accomplish this goal, we propose to use next generation sequencing technology to study the nematode side of the pathosystem. In particular we aim to focus on the genes encoding nematode proteins that are secreted in planta during the initial infection processes and compare the transcriptional profiles of the nematodes after infection of a susceptible plant and an auxin-insensitive mutant with enhanced nematode resistance. We will then use gene silencing and initiate a novel effector screen to functionally characterize the roles of these nematode genes during parasitism. The information derived from this proposal will provide the foundations to engineer novel nematode resistance in agriculturally important crop plants.

植物寄生线虫对世界上许多主要粮食作物都是一个巨大的农业问题。其中最具破坏性的植物寄生线虫是根结线虫。根结线虫寄主范围广，缺乏天然植物抗性，是严重的农业威胁。在敏感互作过程中，根结线虫侵入寄主根系，选择植物细胞转化为代谢活跃的取食场所。根结线虫对植物细胞的操纵，特别是线虫如何能够调节寄主植物的途径，尚不清楚。本提案的目的是进一步探索植物/根结线虫的相互作用。我们希望鉴定和表征对成功寄生至关重要的线虫基因，因为这些基因将成为线虫控制的绝佳靶点。为了实现这一目标，我们建议使用下一代测序技术来研究线虫侧的病理系统。特别是，我们的目标是关注编码线虫蛋白的基因，这些蛋白在植物初始感染过程中分泌，并比较易感植物和生长素不敏感突变体感染后线虫的转录谱，这些突变体具有增强的线虫抗性。然后，我们将使用基因沉默和启动一种新的效应筛选来功能表征这些线虫基因在寄生过程中的作用。从该提案中获得的信息将为在重要的农业作物中设计新型线虫抗性提供基础。