Acquisition of a Centrifuge, HPLC System and UV/Visible Spectrophotometer

在过去的几年里，迈阿密生物化学部的研究活动水平急剧提高。该部门增加了一名新的教员（一个新的职位），并获得了975，000美元的研究资金，其中包括来自NSF的四笔赠款。研究活动的增加表明，各司的手段存在若干不足之处。几个受资助的研究项目需要该司目前不具备的能力。所需仪器包括：超高速离心机和转子，具有分离μ g定量蛋白质的微孔能力的梯度HPLC系统，以及具有高灵敏度的扫描UV/可见光分光光度计。所有三件设备，通常在生物化学实验室，是必不可少的至少两个PI，并将偶尔使用的其他三个小组在该司。所要求的仪器将用于支持和加强以下研究项目：1）植物线粒体生物发生和花粉发育的发育调控，2）植物脂肪酸的代谢调控和生物合成，3）单宁在植物-草食动物相互作用中的作用。

In the last several years the level of research activityof the Miami Biochemistry division has increased dramatically. The division has added a new faculty member (a new position) and obtained $975,000 in research funding including four grants from NSF. This increase in research activity has identified several inadequacies in the divisions' instrumentation. Several o fthe funded research projects require capabilities not currently available in the division. The required instrumentation are: a supraspeed centrifuge and rotors, a gradient HPLC system with microbore capabilities to isolate ug quantitites of protein, and a scanning UV/visibile spectrophotometer with high sensitivity. All three pieces of equipment, commonly found in biochemistry laboratories, are essential to at least two of the PIs and will be used occasionally by the other three groups in the division. The requested instruments will be used to support and enhance the following research projects: 1) development regulation of plant mitochondrial biogenesis and pollen development, 2) metabolic regulation and biosynthesis of fatty acids in plant, and 3) the role of tannins in plant-herbivore interactions.