Acquisition of a MALDI-TOF/TOF MS Spectrometer

购买 MALDI-TOF/TOF MS 光谱仪

• 批准号: 0839233
• 负责人: Christopher Makaroff
• 金额: $ 32.66万
• 依托单位: Miami University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0839233&HistoricalAwards=false
• 关键词: Acquisition; MALDI; TOF; MS; Spectrometer

With this award from the Chemistry Research Instrumentation and Facilities: Multiuser program (CRIF:MU), Christopher A. Makaroff and colleagues Michael W. Crowder, Ann E. Hagerman, Christopher S. Hartley, Gary A. Lorigan from Miami University Oxford Campus will acquire a matrix assisted laser desorption/ionization, time-of-flight mass spectrometer with TOF/TOF capability (MALDI-TOF/TOF MS). This instrument will support research from five primary researchers as well as at least nine additional researchers from the departments of Chemistry and Biochemistry, Botany, Zoology and Microbiology. Major research projects will study membrane proteins and peptides, biological antioxidants, ribosomal proteins and proteins involved in zinc transport, protein interactions as well as graphene nanoribbons.MALDI-TOF-mass spectrometry is the technique of choice for obtaining accurate molecular weights for very large molecules, with extremely high sensitivity. Use of a MALDI-TOF mass spectrometer has therefore become a standard technique, especially in studies involving biomolecules. This research will have a significant impact in a number of areas, especially chemistry and biochemistry. This instrument will be an integral part of teaching as well as research.

凭借化学研究仪器和设施多用户计划(CRIF：MU)的这一奖项，来自迈阿密大学牛津校区的Christopher A.Makaroff及其同事Michael W.Crowder、Ann E.Hagerman、Christopher S.Hartley、Gary A.Lolian将获得具有飞行时间/飞行时间能力的基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF/TOF MS)。该仪器将支持来自化学和生物化学、植物学、动物学和微生物学系的5名主要研究人员以及至少9名额外研究人员的研究。主要研究项目将包括膜蛋白和多肽、生物抗氧化剂、核糖体蛋白和锌转运蛋白、蛋白质相互作用以及石墨烯纳米带。MALDI-TOF-MS是获得超大分子准确分子量的首选技术，具有极高的灵敏度。因此，使用MALDI-TOF质谱计已成为一种标准技术，特别是在涉及生物分子的研究中。这项研究将在许多领域产生重大影响，特别是化学和生物化学。该仪器将成为教学和研究不可或缺的一部分。