Acquisition of a Triple Quadrupole Mass Spectrometer

9201814申请Amster基金用于购买一台扩展质量范围的三重四极质谱仪。该仪器将被化学和生物化学系的研究人员用于测定大小在1,000-100,000 Da范围内的肽和蛋白质的分子量，蛋白质的初级结构，以及天然和修饰蛋白质中金属原子的数量和位置。所要求的光谱仪将有助于表征转化生长因子E、吡哆醛-5'磷酸结合位点、生物电子转移途径以及一系列天然和突变的金属酶和金属蛋白。拟议的仪器将用于与佐治亚大学过渡金属在生物学中的作用的国家科学基金会研究训练组直接相关的培训和研究。

9201814 Amster Funds are requested for the purchase an extended mass range triple quadrupole mass spectrometer. This instrument will used by investigators in the Departments of Chemistry and Biochemistry to determine the molecular weights of peptides and proteins with sizes in the range 1,000-100,000 Da, the primary structures of proteins, and the number and location of metal atoms in native and modified proteins. The requested spectrometer will facilitate characterization of transforming growth factor E, pyridoxal-5' phosphate binding sites, biological electron transfer pathways, and a range of native and mutated metalloenzymes and metalloproteins. The proposed instrument will be used for training and research directly related to the NSF research training group in the role of transition metals in biology at the University of Georgia.