Improving Mass Accuracy in FTICR Mass Spectrometry

提高 FTICR 质谱的质量准确度

• 批准号: 9974579
• 负责人: I. Jonathan Amster
• 金额: $ 27.64万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2003-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9974579&HistoricalAwards=false
• 关键词: Improving; Mass; Accuracy; FTICR; Spectrometry

This project, carried out by Professor Jon Amster and his students, and supported by Analytical and Surface Chemistry, involves performance evaluation and analytical methods development with a 4.7 Tesla Fourier Transform mass spectrometer. The project develops a complete understanding of the space charge phenomenon in the cell of an FT ICR instrument, and documents its effect on the mass measurement accuracy. The space charge phenomenon is modeled by a new set of sophisticated computations, and experiments are carried out by changes in the physical design of the cell and the potentials applied to it. High mass measurement accuracy for protein-derived ions is used as an exemplary application to emphasize improvements in performance.The analysis cell of a Fourier Transform ion cyclotron resonance mass spectrometer can only contain a certain number of ions before the ions begin to interact with each other (the space charge effect). The space charge effect limits the mass measurement accuracy of the instrument. This project of Professor Jon Amster of the University of Georgia models the space charge effect in calculation and experiment, and through enlightened instrument design, minimizes its effect on highly charged ions. The result is much improved mass measurement accuracy needed for biomolecular analysis.

该项目由Jon Amster教授和他的学生实施，并得到分析和表面化学的支持，涉及使用4.7特斯拉傅里叶变换质谱仪进行性能评估和分析方法开发。该项目对FT ICR仪器单元中的空间电荷现象有了全面的了解，并记录了它对质量测量精度的影响。空间电荷现象是通过一组新的复杂的计算来模拟的，实验是通过改变电池的物理设计和施加到它的电势来进行的。蛋白质衍生离子的高质量测量精度被用作强调性能改进的典型应用。傅立叶变换离子回旋共振质谱仪的分析单元在离子开始相互作用之前只能包含一定数量的离子(空间电荷效应)。空间电荷效应限制了仪器的质量测量精度。佐治亚大学的Jon Amster教授的这个项目在计算和实验中模拟了空间电荷效应，并通过开明的仪器设计，将其对高电荷态离子的影响降至最低。其结果是大大提高了生物分子分析所需的质量测量精度。