Laser Desorption Fourier Transform Mass Spectroscopy Analysis of Peptides

肽的激光解吸傅里叶变换质谱分析

• 批准号: 9412334
• 负责人: I. Jonathan Amster
• 金额: $ 24.5万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9412334&HistoricalAwards=false
• 关键词: Laser; Desorption; Fourier; Transform; Mass

This work, supported in the Analytical and Surface Chemistry Program, is focused on the development of Fourier transform - ion cyclotron resonance mass spectrometry for the structural characterization of peptides in the 1,000 to 10,000 Dalton molecular weight range. Laser desorption of neutral species, followed by chemical ionization, is used to generate the ions, which are then analyzed using tandem mass spectrometry. Techniques that involve refocusing the ions in the center of the ion cyclotron cell will be developed to improve sensitivity, and different chemical ionization reagents, including metal ions, will be explored to provide maximum information about the peptide sequence. Professor Amster and his graduate students at the University of Georgia will investigate improved methods for the characterization of peptides using mass spectrometry. Reactions between laser desorption molecules and gas-phase ionic reagents will be studied. Advances in this area have important implications for biotechnology, including the characterization of new peptide drugs.

这项工作得到了分析和表面化学计划的支持，重点是开发傅立叶变换-离子回旋共振质谱仪，用于1,000至10,000道尔顿分子量范围内的多肽的结构表征。中性物质的激光解吸，然后是化学电离，产生离子，然后用串联质谱仪进行分析。将开发涉及离子回旋加速器中心离子重新聚焦的技术以提高灵敏度，并将探索不同的化学电离试剂，包括金属离子，以提供关于肽序列的最大信息。阿姆斯特教授和他在佐治亚大学的研究生将研究利用质谱学表征多肽的改进方法。将研究激光解吸分子与气相离子试剂之间的反应。这一领域的进展对生物技术，包括新的多肽药物的表征具有重要意义。