Laser Desorption Fourier Transform Mass Spectroscopy Analysis of Peptides

这项工作，在分析和表面化学计划的支持，是 重点发展傅里叶变换-离子回旋共振 质谱法用于肽的结构表征 1，000至10，000道尔顿分子量范围。 激光解吸 中性物质，随后是化学电离，用于产生 离子，然后使用串联质谱法进行分析。 技术 包括在离子回旋加速器单元的中心重新聚焦离子 将被开发以提高灵敏度，不同的化学物质 将探索包括金属离子在内的电离试剂， 关于肽序列的最大信息。 阿姆斯特教授和他在格鲁吉亚大学的研究生们 将研究用于表征肽的改进方法 使用质谱法。 激光解吸分子间的反应 和气相离子试剂进行研究。 这一领域的进展 对生物技术的重要影响，包括 新的多肽药物。

This work, supported in the Analytical and Surface Chemistry Program, is focused on the development of Fourier transform - ion cyclotron resonance mass spectrometry for the structural characterization of peptides in the 1,000 to 10,000 Dalton molecular weight range. Laser desorption of neutral species, followed by chemical ionization, is used to generate the ions, which are then analyzed using tandem mass spectrometry. Techniques that involve refocusing the ions in the center of the ion cyclotron cell will be developed to improve sensitivity, and different chemical ionization reagents, including metal ions, will be explored to provide maximum information about the peptide sequence. Professor Amster and his graduate students at the University of Georgia will investigate improved methods for the characterization of peptides using mass spectrometry. Reactions between laser desorption molecules and gas-phase ionic reagents will be studied. Advances in this area have important implications for biotechnology, including the characterization of new peptide drugs.