Chemical Ionization of Laser Desorbed Peptides in a Fourier Transform Mass Spectrometer

傅里叶变换质谱仪中激光解吸肽的化学电离

• 批准号: 9024922
• 负责人: I. Jonathan Amster
• 金额: $ 19.66万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1994-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9024922&HistoricalAwards=false
• 关键词: Chemical; Ionization; Laser; Desorbed; Peptides

This research, supported by the Analytical and Surface Chemistry Program in the Chemistry Division, will investigate ways to determine the sequence of fundamental building blocks of small proteins using a variant of mass spectrometry. Potential applications include diagnosis of some genetic diseases, and monitoring of metabolic irregularities. %%% Research, supported by the Analytical and Surface Chemistry Program in the Chemistry Division, will use laser desorption mass spectrometry and collision-induced dissociation to study the fragmentation of vaporized peptides. Fourier Transform mass spectrometry will allow many sequential collision/measurement cycles, so that one may step-wise decompose the peptides. Both atomic and molecular collision species will be tested.

这项研究得到了分析和表面的支持， 化学系的化学课程，将调查 确定基本构件顺序的方法 小蛋白质的质谱分析。 潜在的应用包括诊断一些遗传 疾病和监测代谢异常。 %%% 研究，由分析和表面化学支持 在化学部的程序，将使用激光解吸 质谱和碰撞诱导解离来研究 蒸发的肽的片段化。 傅里叶变换 质谱法将允许许多连续的 碰撞/测量周期，以便可以逐步 分解肽。 原子和分子碰撞 物种将被测试。