Microlithographic Manipulation of Macromolecules

微光刻准二维复杂表面将被构建，DNA将被电泳。大单分子通过这些障碍阵列的运动将使用实时荧光成像技术进行观察。这些设备应该允许在高度可复制的环境中，在染色体长度尺度上分离DNA分子。通过合成微光刻表面结合和移动非常长的DNA分子的能力可用于在复杂的设计表面上排列染色体长度的DNA分子，使用扫描技术分析DNA序列，并使用光学传感器探测DNA结构。该项目的总体目标是单分子处理。

Microlithographic quasi-two-dimensional complex surfaces will be constructed through which DNA will be electrophoresed. The motions of large single molecules through these obstacle arrays will be observed using real-time fluorescence imaging techniques. %%% These devices should allow the fractionation of DNA molecules at the chromosome length scale in a highly reproducible environment. The ability to bind and move very long DNA molecules through synthetic microlithographic surfaces may be used to align chromosomal length DNA molecules on complex designed surfaces, to analyze the DNA sequence using scanning techniques and to probe DNA structure using optical sensors. The overall aim of the project is single molecule processing.