Microlithographic Manipulation of Macromolecules

微平版印刷术将构建准二维复杂表面，DNA将通过该表面进行电泳化。大分子通过这些障碍阵列的运动将使用实时荧光成像技术进行观察。这些设备应该允许在高度可重复性的环境中在染色体长度范围内对DNA分子进行分离。通过合成微影刻面结合和移动超长DNA分子的能力可用于在复杂的设计表面上排列染色体长度的DNA分子，使用扫描技术分析DNA序列，以及使用光学传感器探测DNA结构。该项目的总体目标是单分子加工。

Microlithographic quasi-two-dimensional complex surfaces will be constructed through which DNA will be electrophoresed. The motions of large single molecules through these obstacle arrays will be observed using real-time fluorescence imaging techniques. %%% These devices should allow the fractionation of DNA molecules at the chromosome length scale in a highly reproducible environment. The ability to bind and move very long DNA molecules through synthetic microlithographic surfaces may be used to align chromosomal length DNA molecules on complex designed surfaces, to analyze the DNA sequence using scanning techniques and to probe DNA structure using optical sensors. The overall aim of the project is single molecule processing.