Microlithographic Manipulation of Macromolecules

微光刻准二维复杂表面将是 构建DNA将通过其进行电泳。 的 大的单分子通过这些障碍物阵列的运动 将使用实时荧光成像技术进行观察。 %%% 这些装置应该允许DNA分子的分级分离， 染色体长度尺度在一个高度可重复的环境。 结合并移动很长的DNA分子的能力 合成微光刻表面可用于对准 染色体长度的DNA分子在复杂的设计表面， 使用扫描技术分析DNA序列， 使用光学传感器的DNA结构。 的总体目标 项目是单分子加工。

Microlithographic quasi-two-dimensional complex surfaces will be constructed through which DNA will be electrophoresed. The motions of large single molecules through these obstacle arrays will be observed using real-time fluorescence imaging techniques. %%% These devices should allow the fractionation of DNA molecules at the chromosome length scale in a highly reproducible environment. The ability to bind and move very long DNA molecules through synthetic microlithographic surfaces may be used to align chromosomal length DNA molecules on complex designed surfaces, to analyze the DNA sequence using scanning techniques and to probe DNA structure using optical sensors. The overall aim of the project is single molecule processing.