Bioactive Material Template Surfaces for in Vitro Synthesis of Bone Tissue

该研究项目的重点是 体外组织工程 生长的钙化组织 它将 研究合成 生物活性陶瓷模板 骨和骨的最佳形成- 就像组织。 本研究旨在 在解释陶瓷 底物的活性 骨祖细胞和 成骨细胞表型 的 下列各项之间的关系 时间相关参数将是 （1）形态学 和成分特性 细胞外组织 的形成与表达 成骨细胞表型; 2） 显微镜和 超显微组成和 生物活性玻璃结构 表面使用FTIR，AES，ESCA和 AFM; 3）时间依赖性 细胞外液变化 成分和流体流动接近 玻璃表面;以及4）zeta 生物活性玻璃的潜力 面 这项研究很重要，因为它 可以促进新的领域， 体外组织工程 的 研究可以改善 在材料的质量， 替换或修复骨骼。

This research project is focused on tissue engineering of in vitro grown calcified tissues. It will investigate the synthesis of bioactive ceramic templates for optimum formation of bone and bone- like tissue. This study is aimed at explaining the effect of ceramic substrates on the activity of osteoprogenitor cells and cells of osteoblastic phenotype. The relationship among the following time dependent parameters will be established: 1) the morphological and compositional characteristics of the extracellular tissue formation and the expression of osteoblastic phenotype; 2) the microscopical and ultramicroscopical composition and structure of the bioactive glass surface using FTIR, AES, ESCA and AFM; 3) the time dependent variation of extracellular fluid composition and fluid flow close to the glass surface; and 4) the zeta potential of the bioactive glass surface. This research is important since it could promote the new field of in vitro tissue engineering. The research could lead to improvements in the quality of materials to replace or repair bone.