Bioactive Material Template Surfaces for in Vitro Synthesis of Bone Tissue

本课题主要研究体外培养钙化组织的组织工程。它将研究生物活性陶瓷模板的合成，以优化骨和骨样组织的形成。本研究旨在解释陶瓷基质对成骨细胞和成骨表型细胞活性的影响。将建立如下时变参数之间的关系：1)细胞外组织形成的形态组成特征及成骨细胞表型的表达；2)利用FTIR、AES、ESCA和AFM分析生物活性玻璃表面的微观和超微组成和结构；3)细胞外液组成和靠近玻璃表面的流体流动随时间的变化；4)生物活性玻璃表面的zeta电位。本研究对促进体外组织工程的发展具有重要意义。这项研究可能会提高替代或修复骨骼材料的质量。

This research project is focused on tissue engineering of in vitro grown calcified tissues. It will investigate the synthesis of bioactive ceramic templates for optimum formation of bone and bone- like tissue. This study is aimed at explaining the effect of ceramic substrates on the activity of osteoprogenitor cells and cells of osteoblastic phenotype. The relationship among the following time dependent parameters will be established: 1) the morphological and compositional characteristics of the extracellular tissue formation and the expression of osteoblastic phenotype; 2) the microscopical and ultramicroscopical composition and structure of the bioactive glass surface using FTIR, AES, ESCA and AFM; 3) the time dependent variation of extracellular fluid composition and fluid flow close to the glass surface; and 4) the zeta potential of the bioactive glass surface. This research is important since it could promote the new field of in vitro tissue engineering. The research could lead to improvements in the quality of materials to replace or repair bone.