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Molecular Genetic Techniques: Testing Reproductive Success (Career Advancement Award)

分子遗传技术：测试生殖成功（职业进步奖）

基本信息

批准号：
9209475
负责人：
Naida Zucker
金额：
$ 4.95万
依托单位：
New Mexico State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1992
资助国家：
美国
起止时间：
1992-10-15 至 1994-09-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9209475&HistoricalAwards=false
关键词：
Molecular Genetic Techniques Testing Reproductive

项目摘要

One of the most exciting recent advances in the field of behavioral ecology is the application of molecular genetic techniques to answer critical questions concerning relative reproductive success of various classes of individuals in populations. Evolutionary interpretations of results of early studies have had to rely on various assumptions about mating patterns, some of which have subsequently been shown to be erroneous using these new techniques. I propose to learn to use these techniques (especially DNA fingerprinting) so that I may answer questions of paternity for studies on the behavioral ecology of lizards, a group of organisms currently under-represented in the behavioral ecology literature yet well-suited to such studies. Reptiles are an important group to include in comparative studies of the evolution of mating systems and social behavior since they represent a bridge between the ectothermic vertebrates and the reproductively terrestrial vertebrates. After learning these techniques at the Genetics Laboratory of the National Zoo, I plan to undertake a behavioral study on alternative mating tactics, followed by paternity analysis. The paternity analysis would take place in the common-use Laboratory of Evolutionary and Ecological Genetics, currently under development in Biology at NMSU, and would test my ability.

行为领域最令人兴奋的最新进展之一生态学是应用分子遗传技术来回答关于相对繁殖成功的关键问题种群中的各种个体。进化对早期研究结果的解释不得不依赖于关于交配模式的各种假设，其中一些假设随后使用这些新技术被证明是错误的。我建议学习使用这些技术（尤其是DNA 指纹），以便我可以回答亲子关系的问题，蜥蜴的行为生态学研究目前在行为生态学文献中的代表性不足，非常适合这样的研究。爬行动物是一个重要的群体，包括交配系统进化的比较研究和社会行为，因为它们代表了变温脊椎动物和陆生生殖动物脊椎动物在遗传学学会这些技术后，国家动物园实验室，我计划进行一项行为研究不同的交配策略，然后进行亲子鉴定。亲子鉴定将在共用实验室进行进化和生态遗传学，目前正在开发中在NMSU的生物学专业，并会测试我的能力。