Development of 750 MHz Spectroscopy at the National Magnetic Resonance Facility at Madison.

为布鲁克AMX-750（17.63特斯拉，750兆赫）核磁共振光谱仪提供50%的资金，该光谱仪将成为威斯康星大学麦迪逊分校生物化学系3000万美元扩建计划的一部分。计划扩建的一个侧翼是为了满足麦迪逊国家磁共振设施（NMRFAM）当前和未来的需求。该中心将与威斯康星大学麦迪逊分校的物理和生物科学研究人员以及来自美国和其他几个国家的其他研究机构的访客使用的现有共享仪器设施相邻。750 MHz核磁共振波谱仪代表了新兴技术。它将包括一个持久的17.63特斯拉超导螺线管，该螺线管具有5厘米的室温孔径，是布鲁克在1991年开发的那种。与该系统相关的控制台和探头将是最先进技术的代表。容纳该仪器的新建筑将被设计为处理该系统的特殊选址要求。750 MHz核磁共振谱仪的研究时间将根据科学价值分配给竞争项目。NMRFAM的地方和国家咨询委员会将监督其使用情况。NMRFAM有全职的支持人员（包括四名具有博士学位的设施工作人员），他们将提供：用户培训；协助光谱数据采集、处理和分析；日常维护;和维修。NMRFAM在为用户提供新技术，保持仪器处于最佳运行状态，修改或构建特殊电路和探针以及为NMR数据分析创建软件工具方面也具有良好的记录。750 MHz核磁共振光谱仪的用户群包括威斯康星大学麦迪逊分校15个系的40个实验室的研究生、博士后研究助理、工作人员科学家和主要研究人员。此外，位于校外的大约35个团体使用该设施。新建的大楼将为来访的科学家提供空间。750兆赫的光谱仪将用于需要其独特的高场强的实验：研究较大生物分子中的核Overhauser效应，研究化学交换和关系现象的场依赖性，研究需要特殊分辨率和/或灵敏度的蛋白质和其他生物分子的功能。随着这台光谱仪的加入，NMRFAM将处于独特的位置，为用户提供9.4到17.63特斯拉的磁场强度，用于研究场相关现象。NMRFAM的工作人员将制定核磁共振实验方案，以解决这种新型光谱仪将为结构和机械生物学的研究和培训提供的问题和机会。

Funds are requested for 50% funding of a Bruker AMX-750 (17.63 tesla, 750 MHz) NMR spectrometer which will become part of a $30 million planned expansion of the Biochemistry Department, University of Wisconsin-Madison. One wing of the planned building expansion is designed to meet current and future needs of the National Magnetic Resonance Facility at Madison (NMRFAM). This wing will be contiguous with the current shared instrumentation facility which is used by researchers in the physical and biological sciences at the University of Wisconsin-Madison campus and visitors from other research institutions in the United States and several foreign countries. The 750 MHz NMR spectrometer represents newly emerging technology. It will incorporate a persistent 17.63 Tesla superconducting solenoid with 5-cm room- temperature bore of the type developed by Bruker in 1991. The console and probes associated with this system will be representative of the state-of-the-art. The new construction to house this instrument will be designed to handle the special siting requirements of this system. Research time on the 750 MHz NMR spectrometer will be allocated on the basis of scientific merit to competing projects. The local and national advisory committees to NMRFAM will oversee its use. NMRFAM has full-time support staff (including four facility staff members with Ph.D. degrees) who will provide: user training; assistance with spectral data acquisition, processing, and analysis; routine maintenance; and repairs. NMRFAM also has a proven track record for making new technology available to its users, for keeping instrumentation in top operating condition, for modifying or constructing special circuits and probes, and for creating software tools for NMR data analysis. The user base for the 750 MHz NMR spectrometer includes graduate students, postdoctoral research associates, staff scientists, principal investigators form 40 laboratories in 15 departments on the University of Wisconsin-Madison Campus. In addition, the facility is used by about 35 groups located off campus. The new building addition will have space for visiting scientists. The 750 MHz spectrometer will be devoted to experiments that require its uniquely-high field strength: studies of the nuclear Overhauser effect in larger biomolecules, studies of the field dependence of chemical exchange and relation phenomena, functional studies of proteins and other biomolecules that require exceptional resolution and/or sensitivity. With the addition of this spectrometer, NMRFAM will be in the unique position of offering its user magnetic field strengths between 9.4 and 17.63 tesla for studies of field -dependent phenomena. The staff of NMRFAM will develop NMR experimental protocols to deal with the problems and opportunities that this new spectrometer will offer to research and training in structural and mechanistic biology.