Development of 750 MHz Spectroscopy at the National Magnetic Resonance Facility at Madison.

麦迪逊国家磁共振设施开发 750 MHz 光谱仪。

• 批准号: 9214394
• 负责人: John Markley
• 金额: $ 150万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-12-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9214394&HistoricalAwards=false
• 关键词: Development; 750; MHz; Spectroscopy; National

Funds are requested for 50% funding of a Bruker AMX-750 (17.63 tesla, 750 MHz) NMR spectrometer which will become part of a $30 million planned expansion of the Biochemistry Department, University of Wisconsin-Madison. One wing of the planned building expansion is designed to meet current and future needs of the National Magnetic Resonance Facility at Madison (NMRFAM). This wing will be contiguous with the current shared instrumentation facility which is used by researchers in the physical and biological sciences at the University of Wisconsin-Madison campus and visitors from other research institutions in the United States and several foreign countries. The 750 MHz NMR spectrometer represents newly emerging technology. It will incorporate a persistent 17.63 Tesla superconducting solenoid with 5-cm room- temperature bore of the type developed by Bruker in 1991. The console and probes associated with this system will be representative of the state-of-the-art. The new construction to house this instrument will be designed to handle the special siting requirements of this system. Research time on the 750 MHz NMR spectrometer will be allocated on the basis of scientific merit to competing projects. The local and national advisory committees to NMRFAM will oversee its use. NMRFAM has full-time support staff (including four facility staff members with Ph.D. degrees) who will provide: user training; assistance with spectral data acquisition, processing, and analysis; routine maintenance; and repairs. NMRFAM also has a proven track record for making new technology available to its users, for keeping instrumentation in top operating condition, for modifying or constructing special circuits and probes, and for creating software tools for NMR data analysis. The user base for the 750 MHz NMR spectrometer includes graduate students, postdoctoral research associates, staff scientists, principal investigators form 40 laboratories in 15 departments on the University of Wisconsin-Madison Campus. In addition, the facility is used by about 35 groups located off campus. The new building addition will have space for visiting scientists. The 750 MHz spectrometer will be devoted to experiments that require its uniquely-high field strength: studies of the nuclear Overhauser effect in larger biomolecules, studies of the field dependence of chemical exchange and relation phenomena, functional studies of proteins and other biomolecules that require exceptional resolution and/or sensitivity. With the addition of this spectrometer, NMRFAM will be in the unique position of offering its user magnetic field strengths between 9.4 and 17.63 tesla for studies of field -dependent phenomena. The staff of NMRFAM will develop NMR experimental protocols to deal with the problems and opportunities that this new spectrometer will offer to research and training in structural and mechanistic biology.

布鲁克AMX-750(17.63特斯拉，750 MHz)核磁共振光谱仪的50%资金被申请，该光谱仪将成为威斯康星大学麦迪逊分校生化系3000万美元扩建计划的一部分。计划中的建筑扩建的一翼是为了满足麦迪逊国家磁共振设施(NMRFAM)目前和未来的需求。这一侧翼将与目前的共享仪器设施相连，威斯康星大学麦迪逊分校物理和生物科学的研究人员以及来自美国和几个国家的其他研究机构的访问者使用该设施。750 MHz核磁共振波谱仪代表了新兴技术。它将采用布鲁克在1991年开发的具有5厘米室温内径的17.63特斯拉超导螺线管。与该系统相关的控制台和探头将代表最先进的技术。容纳该仪器的新结构将被设计为满足该系统的特殊选址要求。750兆赫核磁共振波谱仪的研究时间将根据竞争项目的科学价值进行分配。NMRFAM的地方和国家咨询委员会将监督其使用。NMRFAM有全职支持人员(包括四名拥有博士学位的设施工作人员)，他们将提供：用户培训；协助获取、处理和分析光谱数据；日常维护；以及维修。NMRFAM在向用户提供新技术、将仪器保持在最佳运行状态、修改或构建特殊电路和探头以及创建用于核磁共振数据分析的软件工具方面也拥有经过验证的记录。750 MHz核磁共振波谱仪的用户群包括威斯康星大学麦迪逊校区15个系的40个实验室的研究生、博士后研究助理、工作人员科学家和首席研究员。此外，该设施还被位于校外的大约35个团体使用。新增加的建筑将为来访的科学家提供空间。750 MHz光谱仪将致力于需要其独特高场强的实验：研究较大生物分子中的核Overhaser效应，研究化学交换和关联现象的场依赖性，蛋白质和其他生物分子的功能研究，需要特殊的分辨率和/或灵敏度。随着这台光谱仪的加入，NMRFAM将处于独特的地位，为其用户提供9.4至17.63特斯拉之间的磁场强度，用于研究依赖于场的现象。NMRFAM的工作人员将开发核磁共振实验方案，以处理这种新的光谱仪将为结构和机械生物学的研究和培训提供的问题和机会。