Acquisition of a 800 MHz NMR Spectrometer for the National Magnetic Resonance Facility at Madison

为麦迪逊国家磁共振设施采购 800 MHz NMR 波谱仪

• 批准号: 0079387
• 负责人: John Markley
• 金额: $ 103.3万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0079387&HistoricalAwards=false
• 关键词: Acquisition; 800; MHz; NMR; Spectrometer

This award will add an 800 MHz NMR spectrometer to the National Magnetic Resonance Facility at Madison (NMRFAM), a shared instrumentation facility. The presence of this instrument will enable researchers to solve new problems and to achieve definitive answers to scientific questions about biomolecular structure-function relationships. The spectrometer will be devoted to experiments that require its uniquely-high field strength: studies of larger molecules, investigations of field-dependent molecular ordering effects, studies of the field dependence of chemical exchange and relaxation phenomena, and functional studies of proteins, nucleic acids, and other biomolecules that require exceptional resolution and/or sensitivity. The instrument will be used by a core group of about 40 principal investigators from all around the country, who work on a variety of problems. The improved resolution and sensitivity of the proposed spectrometer will ease the limitation of low solubility, lower the time requirements (and molecular stability requirements) for biomolecular data collection, and increase the accuracy of NMR measurements. The increased sensitivity of the instrument, coupled with new data acquisition protocols (such as TROSY-based pulse sequences), promise to roll back the molecular weight limit for structure-function investigations and improve the feasibility of solution structures of membrane proteins and functional RNA molecules.The 800 MHz NMR spectrometer will serve as a focal point for hands-on training in biomolecular NMR spectroscopy. On average, the National Magnetic Resonance Facility at Madison trains about 30 new users each year. These generally are graduate students and postdoctoral research associates. The facility regularly hosts visiting scientists who come for periods of one week to one year to receive training in biomolecular NMR spectroscopy. Training is primarily by hands-on activities supervised by the staff of the facility. All new internal and external users are required to receive orientation and training, which is tailored to their previous experience. The large number of users of the facility and the many maintenance tasks required lead to unique training opportunities. Staff members also train graduate students and advanced undergraduates to assist in the performance of routine maintenance and testing of the spectrometers.

该合同将为位于麦迪逊的国家磁共振设施（NMRFAM）增加一台800 MHz核磁共振光谱仪，这是一个共享的仪器设施。该仪器的存在将使研究人员能够解决新的问题，并获得有关生物分子结构-功能关系的科学问题的明确答案。光谱仪将致力于需要其超高场强的实验：较大分子的研究，场依赖性分子有序效应的调查，化学交换和弛豫现象的场依赖性研究，以及蛋白质，核酸和其他需要特殊分辨率和/或灵敏度的生物分子的功能研究。该工具将由来自全国各地的约40名主要调查人员组成的核心小组使用，他们研究各种问题。所提出的光谱仪的改进的分辨率和灵敏度将减轻低溶解度的限制，降低生物分子数据收集的时间要求（和分子稳定性要求），并增加NMR测量的准确性。仪器灵敏度的提高，加上新的数据采集协议（如基于TROSY的脉冲序列），有望降低结构-功能研究的分子量限制，提高膜蛋白和功能RNA分子溶液结构的可行性。800 MHz NMR光谱仪将作为生物分子NMR光谱学实践培训的焦点。平均而言，位于麦迪逊的国家磁共振设施每年培训约30名新用户。这些通常是研究生和博士后研究助理。 该设施定期接待来访的科学家，他们来这里接受生物分子NMR光谱学的培训，为期一周到一年。培训主要是由设施工作人员监督的实践活动。 所有新的内部和外部用户都必须接受根据其以往经验量身定制的情况介绍和培训。该设施的用户众多，所需的维护任务众多，因此提供了独特的培训机会。工作人员还培训研究生和本科生，以协助进行光谱仪的日常维护和测试。