Cryoprobe repairs and amplifier replacement for the 800 and 750 MHz spectrometers

800 和 750 MHz 光谱仪的冷冻探针维修和放大器更换

• 批准号: 10400474
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 8.27万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10400474
• 关键词: 2,4-Dinitrophenol; 3-Dimensional; Agreement; Amplifiers; Binding; Caliber; Cholesterol; Communities; Core Protein; Crystallization; Development; Diamond; Disease; Ensure; Equipment; Evolution; Frequencies; Funding; G-Protein-Coupled Receptors; Grant; Helium; Investigation; Isotope Labeling; Label; Laboratories; Libraries; Magic; Magnetic Resonance; Magnetic Resonance Spectroscopy; Measures; Membrane Proteins; Methods; Molecular; Morphologic artifacts; Motion; Nature; Nuclear; Nucleic Acids; Pharmacologic Substance; Physiologic pulse; Procedures; Production; Protein Dynamics; Proteins; Request for Proposals; Research; Resolution; Sampling; Schedule; Seeds; Side; Signal Transduction; Site; Structure; System; Techniques; Technology; Temperature; Tertiary Protein Structure; Thermal Conductivity; Time; Tyrosine; Vertebral column; base; biological systems; cohort; cost; cryogenics; design; dipole moment; experimental study; improved; instrument; instrumentation; magnetic dipole; magnetic field; microwave electromagnetic radiation; next generation; optimal control theory; protein function; protein structure; repaired; structural biology; theories; transmission process

Project Summary Overall The Overall section of the P41 presents a summary of the four TR&D projects which are part of the proposal. These include TR&D 1 – New instrumentation for magnetic resonance TR&D 2 – High-field and fast-MAS methods for biomolecular structure and dynamics TR&D 3 – Next generation solution NMR methods TR&D 4 -- Advanced Magic Angle Spinning NMR methods The specific aims of each of these projects is described and a summary of the contents in presented in more detail. In addition, we provide a rationale for the P41 center which includes the cost and size of present day instrumentation, the unique requirements for instruments and the sophistication of magnetic resonance spectroscopy. We also provide a table enumerating the equipment presently available for the CMR and a list of the new instrumentation that will be constructed during the five years of the proposed grant.

项目摘要 整体 P41的总体部分概述了四个研发项目， 提案的一部分。这些包括 TR&D 1 -新的磁共振仪器 TR&D 2 -生物分子结构和动力学的高场和快速MAS方法 TR&D 3 -下一代溶液NMR方法 TR&D 4 --高级魔角旋转NMR方法 每一个项目的具体目标都有说明， 更详细地介绍。 此外，我们还提供了P41中心的基本原理，其中包括成本和规模， 当今的仪器，对仪器的独特要求和复杂性， 磁共振波谱学 我们还提供了一个表格，列举了目前可用于CMR的设备， 一份将在拟议的五年内建造的新仪器清单 格兰特.

项目成果

The Cytoplasmic Domain of the SARS-CoV-2 Envelope Protein Assembles into a β-Sheet Bundle in Lipid Bilayers.

• DOI: 10.1016/j.jmb.2023.167966
• 发表时间: 2023-03-01
• 期刊: JOURNAL OF MOLECULAR BIOLOGY
• 影响因子: 5.6
• 作者: Dregni, Aurelio J.;McKay, Matthew J.;Surya, Wahyu;Queralt-Martin, Maria;Medeiros-Silva, Joao;Wang, Harrison K.;Aguilella, Vicente;Torres, Jaume;Hong, Mei
• 通讯作者: Hong, Mei

NMR Model of the Entire Membrane-Interacting Region of the HIV-1 Fusion Protein and Its Perturbation of Membrane Morphology.

• DOI: 10.1021/jacs.1c01762
• 发表时间: 2021-04
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: A. Piai;Q. Fu;Amanda Sharp;B. Bighi;A. M. Brown;J. Chou

High fidelity sampling schedules for NMR spectra of high dynamic range.

• DOI: 10.1016/j.jmr.2022.107228
• 发表时间: 2022-06
• 期刊: JOURNAL OF MAGNETIC RESONANCE
• 影响因子: 2.2
• 作者: Hyberts, Sven G.;Wagner, Gerhard
• 通讯作者: Wagner, Gerhard

Membrane-induced tau amyloid fibrils.

• DOI: 10.1038/s42003-023-04847-6
• 发表时间: 2023-04-28
• 期刊: COMMUNICATIONS BIOLOGY
• 影响因子: 5.9
• 作者: Mammeri, Nadia El;Gampp, Olivia;Duan, Pu;Hong, Mei
• 通讯作者: Hong, Mei

Hexamethylene amiloride binds the SARS-CoV-2 envelope protein at the protein-lipid interface.

• DOI: 10.1002/pro.4755
• 发表时间: 2023-10
• 期刊: Protein science : a publication of the Protein Society