Acquisition of a Videomicroscopy Image Processing System

购买视频显微镜图像处理系统

• 批准号: 9217008
• 负责人: John Schmidt
• 金额: $ 4.53万
• 依托单位: SUNY at Albany
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-15 至 1997-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9217008&HistoricalAwards=false
• 关键词: Acquisition; Videomicroscopy; Image; Processing; System

The proposal involves the acquisition of a videomicroscopy and image analysis system for a group of neurobiolgists and cell biologists whose work focuses on neuronal development and cell adhesion and motility. The components to be acquired were chosen to function together in different flexible combinations to serve the varied needs of the individual research projects. The capabilities include: 1) low light level observation and time lapse analysis of growth and plasticity of living cells using phase contrast, DIC or fluorescent staining, 2) calcium mapping in live neurons and growth cones using the fura-2 fluorescence ratio image method including representation in pseudo color images, 3) recombining of images of objects present in more than one section (in sectioned material) or optical plane of focus (in whole mounts) and generating single clear images of entire 3-D structures, 4) superimposition of images of double (or triple) fluorescent staining, obtained at different wavelengths, in order to better compare the colocalization of immunostaining in cellular structures, 5) contrast enhancement and subtraction of background mottle to permit DIC-video images in real-time, 6) provisions for mass storage, retrieval and printing of the images and the production of short time-lapse videotapes for demonstrations and seminars.

该提案涉及为一群神经生物学家和细胞生物学家购买视频显微镜和图像分析系统，他们的工作重点是神经元发育和细胞粘附和运动。选择要采购的组件以不同的灵活组合一起发挥作用，以满足各个研究项目的不同需求。 这些功能包括：1) 使用相差、DIC 或荧光染色对活细胞的生长和可塑性进行低光水平观察和延时分析，2) 使用 fura-2 荧光比图像方法对活神经元和生长锥进行钙成像，包括以伪彩色图像表示，3) 重新组合存在于多个切片（在切片材料中）或光学聚焦平面（在整个安装中）中的物体图像并生成单个清晰图像 完整的 3-D 结构，4) 叠加在不同波长下获得的双重（或三重）荧光染色图像，以便更好地比较细胞结构中免疫染色的共定位，5) 对比度增强和背景斑驳的扣除，以允许实时 DIC 视频图像，6) 提供大量存储、检索和打印图像以及制作短延时录像带 示威和研讨会。