Acquisition of a Videomicroscopy Image Processing System

该提案涉及购买一台视频显微镜 和图像 分析系统的一组 神经生物学家和细胞生物学家 专注于神经元发育和细胞粘附， 能动性选择要采购的组件， 在不同的灵活组合中共同发挥作用， 满足不同研究项目的不同需求。 其功能包括：1）微光观测， 活细胞生长和可塑性的时间推移分析 使用相差、DIC或荧光染色，2） 钙映射在活神经元和生长锥使用的 fura-2荧光 比率图像方法，包括 表示在伪彩色图像，3）重组 存在于多个部分中的对象的图像（在 截面材料）或光学焦平面（整体 安装），并生成整个3D的单个清晰图像 结构，4）双重（或 三重） 荧光染色，在不同的 为了更好地 比较 细胞结构中免疫染色的共定位，5） 对比 背景增强和减除 斑点允许DIC视频图像实时，6） 大容量存储、检索和打印 图像 以及制作短时间的 录像带用于演示和研讨会。

The proposal involves the acquisition of a videomicroscopy and image analysis system for a group of neurobiolgists and cell biologists whose work focuses on neuronal development and cell adhesion and motility. The components to be acquired were chosen to function together in different flexible combinations to serve the varied needs of the individual research projects. The capabilities include: 1) low light level observation and time lapse analysis of growth and plasticity of living cells using phase contrast, DIC or fluorescent staining, 2) calcium mapping in live neurons and growth cones using the fura-2 fluorescence ratio image method including representation in pseudo color images, 3) recombining of images of objects present in more than one section (in sectioned material) or optical plane of focus (in whole mounts) and generating single clear images of entire 3-D structures, 4) superimposition of images of double (or triple) fluorescent staining, obtained at different wavelengths, in order to better compare the colocalization of immunostaining in cellular structures, 5) contrast enhancement and subtraction of background mottle to permit DIC-video images in real-time, 6) provisions for mass storage, retrieval and printing of the images and the production of short time-lapse videotapes for demonstrations and seminars.