Acquisition of a Confocal Laser Scanning Microscope for Cell and Neurobiologists

该提案涉及为一组细胞，分子和神经生物学家提供共聚焦激光扫描显微镜（CLSM）和图像分析系统，他们的工作重点是细胞粘附和运动，基因表达，蛋白质功能和定位以及神经发育。选择的组件提供1)在可见光谱中对不同的荧光色进行共聚焦荧光成像的能力，2)能够记录与CLSM匹配的DIC图像，以帮助精确定位荧光标记结构，3)能够在标本的多个位置拍摄延时共聚焦图像并自动对焦结构。4)使用视频dic作为评估延时CLSM图像的框架来记录细胞运动的能力，5)存储数字图像并将其传输到第二个工作站进行进一步分析的能力，6)在计算机磁盘上大量存储图像并写入cd的能力，以及7)打印出版质量的彩色和黑白显微照片的能力。

The proposal involves the acquisition of a confocal laser scanning microscope (CLSM) and image analysis system for a group of cell, molecular and neurobiologists whose work focuses on cell adhesion and movement, gene expression, protein function and localization and neural development. The components were chosen to provide 1) capability for confocal fluorescence imaging for different fluorochromes in the visible spectrum, 2) ability to record matching DIC images with the CLSM to assist in precise localization of fluorescent labelled structures, 3) an ability to take time-lapse confocal images at multiple positions in a specimen and autofocus on the structures, 4) an ability to record movements of cells using video-DIC as a framework against which time-lapse CLSM images can be evaluated, 5) an ability to store digital images and transfer them to a second work station for further analysis, 6) a capability for mass storage of images on computer disks and for writing to CDs, and 7) the ability to print publication quality color as well as black and white micrographs.