Ultra Fast Time Resolved Spectrofluorometer

提出了一种快速、高灵敏度的时间分辨荧光光谱仪的商业化发展方向。与时间相关单光子计数和相位调制等经典荧光测量方法不同，该仪器基于两项最新发展的技术：a)单脉冲激光激发和电子成像纳秒波形分析；b)声光可调谐滤光器作为电控单色器。这些仪器有能力同时获取一套完整的衰减和光谱曲线，足以在1秒内构建三维流形。仪器的灵敏度可高达飞摩尔(10-15摩尔)和全摩尔(10-18摩尔)水平。这些远远超出了当前工具的能力。该仪器结构紧凑、重量轻、成本低，适合于荧光法的广泛应用。第一阶段将展示可行性，第二阶段将开发原型。长期目标是使用这种方法开发许多专门的仪器，用于基础研究以及临床和工业应用。

A fast and high sensitivity time-resolved spectrofluorometer is proposed to develop into a commercial project. In contrast with the classical fluorometric method, such as time-correlated single photon counting and phase modulation, the novel instrument is based on the introduction of two recently developed techniques: a) single pulse laser excitation and nanosecond waveform analyzing by electronic imaging, and b) acoustooptical tunable filter as an electronically controlled monochromator. The instruments have the capability of simultaneously acquiring a complete set of decay and spectral curve sufficient for the construction of a 3-D manifold within 1 second. The sensitivity of the instrument can be as high as at femtomole (10-15 mole) and attomole (10-18 mole) level. These are far beyond the capabilities of current instruments. The instrument is compact, lightweight, low-cost, suitable for and will facilitate wide applications of the fluoroscopic method. Phase I will demonstrate the feasibility and Phase II will develop a prototype. The long-term goal is to use the method to develop numerous specialized instruments for basic research as well as for clinical and industrial applications.