International Postdoctoral Fellows Program: In Vitro Study of the Role of Vitreoscilla Hemoglobin on the Enhanced Protein Production of Escherichia coli(Travel Award)

国际博士后项目：玻璃颤菌血红蛋白增强大肠杆菌蛋白质生产作用的体外研究（旅行奖）

• 批准号: 9301183
• 负责人: Claire Komives
• 金额: $ 3.83万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9301183&HistoricalAwards=false
• 关键词: International; Postdoctoral; Fellows; Program; Vitro

This award is under the International Postdoctoral Fellows Program, which enables U.S. scientists and engineers to conduct three to twelve months of research at foreign centers of proven excellence. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. Claire Komives of the University of Pittsburgh to work with Professor Dr. J. E. Bailey at the Swiss Federal Institute of Technology in Zurich, Switzerland. The objective of this project is to elucidate the mechanism of Vitreoscilla Hemoglobin (VHb) in vitro, and to study its function in a synthetic cell environment. Two mechanisms for the protein action have been suggested which provide the basis of this study. The capacity of VHb for improving oxygen uptake to the cell, and its potential role in affecting the efficiency of oxygen utilization by specific cell components will be explored. Because VHb both associates with the cell membrane and resides within the cell, its function cannot be properly understood by merely studying it in solution. Reversed micelles offer a novel approach to creating a cell-like environment for carrying out the in vitro mechanistic studies. The surfactant or phospholipid layer encapsulating water pools within an apolar solvent is analogous to a cell membrane. Thus, the action of VHb is to be studied in a well characterized medium that mocks some important features of cell make-up, but which lacks the complexity of interconnected chemical pathways necessary to the function of a living cell. The award recommendation provides funds to cover international travel to Switzerland and a stipend for twelve months.

该奖项是在国际博士后研究员计划下的，该计划使美国科学家和工程师能够在外国卓越中心进行三到十二个月的研究。 该计划的奖项为联合研究提供了机会，以及在国外使用独特或补充设施，专业知识和实验条件。 该奖项将支持匹兹堡大学克莱尔·科米夫斯（Claire Komives）博士在瑞士瑞士瑞士瑞士联邦技术学院的J. E. Bailey博士的12个月后研究访问。 该项目的目的是阐明体外玻璃体血红蛋白（VHB）的机制，并在合成细胞环境中研究其功能。 已经提出了两种蛋白质作用的机制，这些机制提供了本研究的基础。 VHB能够改善对细胞的氧气吸收的能力，以及其在影响特定细胞成分利用氧气效率方面的潜在作用。 由于VHB都与细胞膜相关并驻留在细胞内，因此仅通过在溶液中研究其功能就无法正确理解其功能。 反向胶束提供了一种新的方法来创建类似细胞的环境，以进行体外机理研究。 将水池包裹在溶剂中的表面活性剂或磷脂层类似于细胞膜。 因此，应在嘲笑细胞构成的一些重要特征的培养基中研究VHB的作用，但缺乏活细胞功能所需的相互联系的化学途径的复杂性。 该奖项建议提供了涵盖瑞士国际旅行的资金和十二个月的津贴。