RUI: A Genetic Analysis of Development During Tetrahymena Conjugation

RUI：四膜虫接合过程中发育的遗传分析

• 批准号: 9303456
• 负责人: Eric Cole
• 金额: $ 3.35万
• 依托单位: Saint Olaf College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9303456&HistoricalAwards=false
• 关键词: RUI; Genetic; Analysis; Development; During

9303456 Cole Newly isolated mutations that cause specific lesions during conjugal development in Tetrahymena thermophila will be characterized by fluoresence and immunofluorescence microscopy in combination with double-mutant analysis. The analysis should identify significant developmental checkpoints as well as the necessary and sufficient conditions for passing such checkpoints.The biochemical control of chromatin condensation will be studied by the use of conjugal-block mutants in collaboration with Dr. David Allis. Conjugation mutations that produce 100% penetrant conjugal-arrest phenotypes will be used to isolate secon- site suppressor mutations in order to expand the genetic base of our investigation. As possible, developmentally important genes which complement conjugal-block phenotypes will be cloned. %%% Tetrahymna thermophila is a genetically complex but approachable experimental system which can be induced to undergo conjugation between two compatible mating types. This process will be dissected by genetic and microscopic analysis and, when possible, developmentally important genes will be cloned and preliminarily characterized. Undergraduate students will be involved in the research. ***

9303456科尔在嗜热四膜虫的接合发育过程中引起特异性损伤的新分离的突变将通过荧光和免疫荧光显微镜结合双突变体分析来表征。分析应确定重要的发育检查点以及通过这些检查点的必要和充分条件。染色质凝聚的生化控制将与大卫阿利斯博士合作，通过使用共轭阻断突变体进行研究。将使用产生100%渗透接合停滞表型的接合突变来分离第二位点抑制突变，以扩大我们研究的遗传基础。将尽可能克隆补充接合阻断表型的发育重要基因。 嗜热四膜虫是一个遗传复杂但易于诱导的实验系统，它可以在两个相容的交配型之间进行接合。这一过程将通过遗传和显微镜分析进行解剖，如果可能，将克隆发育重要的基因并进行初步鉴定。本科生将参与研究。 ***