RUI: A Genetic Analysis of Development During Tetrahymena Conjugation

在嗜热四膜虫（Tetrahymena thermophila）的配偶发育过程中，新分离的突变引起特定病变，将通过荧光和免疫荧光显微镜结合双突变分析进行表征。分析应确定重要的发展检查点以及通过这些检查点的必要和充分条件。染色质凝聚的生化控制将通过使用偶联阻滞突变体与大卫·阿利斯博士合作进行研究。产生100%渗透共轭阻滞表型的偶联突变将用于分离第二位点抑制突变，以扩大我们研究的遗传基础。如有可能，发育上重要的基因，补充偶联阻滞表型将被克隆。嗜热四鳃蝇是一种遗传复杂但易于接近的实验系统，它可以在两种相容的交配类型之间诱导进行交配。这一过程将通过遗传和显微分析进行解剖，并在可能的情况下，克隆发育重要基因并初步表征。本科生将参与研究。＊＊＊

9303456 Cole Newly isolated mutations that cause specific lesions during conjugal development in Tetrahymena thermophila will be characterized by fluoresence and immunofluorescence microscopy in combination with double-mutant analysis. The analysis should identify significant developmental checkpoints as well as the necessary and sufficient conditions for passing such checkpoints.The biochemical control of chromatin condensation will be studied by the use of conjugal-block mutants in collaboration with Dr. David Allis. Conjugation mutations that produce 100% penetrant conjugal-arrest phenotypes will be used to isolate secon- site suppressor mutations in order to expand the genetic base of our investigation. As possible, developmentally important genes which complement conjugal-block phenotypes will be cloned. %%% Tetrahymna thermophila is a genetically complex but approachable experimental system which can be induced to undergo conjugation between two compatible mating types. This process will be dissected by genetic and microscopic analysis and, when possible, developmentally important genes will be cloned and preliminarily characterized. Undergraduate students will be involved in the research. ***