Acquisition of: A Cytophotometry Facility for Direct Measurement of Nuclear DNA Content

获得：用于直接测量核 DNA 含量的细胞光度测定设备

• 批准号: 9413759
• 负责人: Eric Cole
• 金额: $ 4.12万
• 依托单位: Saint Olaf College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-10-01 至 1996-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9413759&HistoricalAwards=false
• 关键词: Acquisition; Cytophotometry; Facility; Direct; Measurement

ABSTRACT It is our intention to establish a cytophotometry facility in the St. Olaf Biology Department for both research and training purposes. Such a facility requires a microscope equipped with a light source and filters that allow for absorbency measurements at two wavelengths, a microscope image processor, and a photometer. A cytophotometer will allow us perform accurate, quantitative measurements of nuclear DNA content. This capability will greatly expand upon our research efforts to analyze nuclear and chromosomal behavior during both normal and aberrant conjugation in the ciliated protozoan Tetrahvmena thermophila. Specifically, we will be better able to characterize the nuclear behavior of conjugation mutants that affect both meiotic and mitotic divisions, as well as the differentiation of newly formed polyploid macronuclei.

摘要 我们打算在圣奥拉夫生物系建立一个细胞光度测量设施，用于研究和培训目的。这样的设施需要配备有光源和过滤器，允许在两个波长，显微镜图像处理器和光度计的吸光度测量的显微镜。 细胞光度计将使我们能够对细胞核DNA含量进行准确的定量测量。这种能力将大大扩展我们的研究工作，分析核和染色体的行为在正常和异常的接合在纤毛虫原生动物嗜热Tetrahvmena。具体来说，我们将能够更好地表征核行为的接合突变体，影响减数分裂和有丝分裂，以及分化的新形成的多倍体大核。