A Genetic Dissection of Ciliate Conjugation

纤毛虫接合的遗传解剖

• 批准号: 9507285
• 负责人: Eric Cole
• 金额: $ 30万
• 依托单位: Saint Olaf College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9507285&HistoricalAwards=false
• 关键词: Genetic; Dissection; Ciliate; Conjugation

Cole 9507285 The goal of this research is to initiate a cellular and genetic analysis of development during Tetrahymena conjugation. To that end, we will, 1.) Determine developmental dependencies during conjugation. by a combination of isolating mutants or/and physically manipulating the mating conditions, 2.) Search for developmental checkpoints by searching for mutations that relieve the developmental dependency, and 3.) Identify and characterize the functions of gene products that participate in specific conjugal events. %%% The objective of this research is to understand the cellular and molecular mechanisms by which Tetrahymena thermophila, a species of ciliated protozoan, controls its passage through the complex cytoplasmic and nuclear events associated with mating. Mutants relevant to this process will be isolated and characterized. Also the mating conditions will be physically manipulated. ***

本研究的目的是对四膜虫偶联过程中的发育进行细胞和遗传分析。为此，我们将：确定结合过程中的发育依赖性。通过分离突变体或/和物理操纵交配条件的结合，2 .通过寻找减轻发育依赖性的突变来寻找发育检查点。鉴定和描述参与特定配偶事件的基因产物的功能。本研究的目的是了解嗜热四膜虫是一种纤毛原生动物，通过与交配相关的复杂细胞质和核事件来控制其通道的细胞和分子机制。与此过程相关的突变体将被分离和表征。此外，交配条件将被物理操纵。＊＊＊