Using Cobalt to Probe DNA and RNA Folding

这个由有机动力和特殊项目项目颁发的女性职业进步奖支持纽约州立大学石溪分校辛西娅·j·巴罗斯教授的工作。这项工作涉及使用DNA和RNA靶标对简单钴化合物的氧化反应进行调查，作为研究者使用镍（II）配合物进行DNA氧化研究的延伸。特别是含有G-G不匹配、G凸起或发夹环的DNA序列将被研究，以开发用于此类结构的探针。RNA的研究将包括检查来自四膜虫的转移RNA和I组内含子。本工作的实验方法将集中在DNA和RNA的高分辨率凝胶电泳纯化。此次授予Burrows教授的职业成就奖将把她在镍配合物及其与DNA相互作用方面的工作扩展到钴配合物的使用以及RNA结构的操纵和催化。这些非卟啉复合物在DNA和RNA上的应用是在双重错配的DNA、转移RNA和来自四膜虫的I族内含子中寻求的。这项工作预计将对医学、协调和生物化学产生影响。

This Career Advancement Award for Women from the Organic Dynamics and Special Projects Programs supports the work of Professor Cynthia J. Burrows at the State University of New York, Stony Brook. The work concerns an investigation into the oxidative reactions of simple cobalt compounds using DNA and RNA targets as an extension of the investigator's DNA oxidation studies using nickel(II) complexes. In particular, DNA sequences containing G-G mismatches, G bulges or hairpin loops will be studied in efforts to develop a probe for such structures. Studies on RNA will include examination of transfer RNA's and the group I intron from Tetrahymena. The experimental methods of this work will focus on DNA and RNA purification by high resolution gel electrophoresis. %%% This Career Advancement Award to Professor Burrows will extend her work on nickel complexes and their interactions with DNA to use of cobalt complexes and to RNA structure manipulations and catalysis. The application of these nonporphyrin complexes to both DNA and RNA is sought in DNA with double mismatches, transfer RNA, and group I intron from Tetrahymena. The work is expected to impact on medical, coordination, and biochemistry.