Determination of the Molecular Basis of Biological Recognition at Functionalized Interfaces

通过这个项目， 支撑分子影响 受体结合表面 速率和结合亲和力 固定化配体将被 阐明。 动力学模型 受体结合有待开发 其中包含分子 互动的细节。 的 动力学和平衡结合 功能化表面的受体 将研究：（1）瞬逝 光纤荧光 光谱学;（2）定量 荧光显微镜;和（3） 放射性标记 受体吸附 解吸率和结合 亲和力将由 渐逝光纤光谱学 特异性受体的均一性 将通过以下方式评估约束力： 荧光显微镜 结合 对两种官能化的 平坦的表面和囊泡将 用放射性标记的 proteins.

With this project, the precise molecular influences of supporting surfaces on receptor association rates and binding affinities to immobilized ligands are to be elucidated. Kinetic models of receptor binding are to be developed which incorporate the molecular details of interactions. The kinetics and equilibrium binding of receptors to functionalized surfaces will be studied by: (1) evanescent fiber optic fluorescence spectroscopy; (2) quantitative fluorescence microscopy; and (3) radiolabelling. Receptor adsoption and desorption rates and binding affinities will be determined by evanescent fiber optic spectroscopy. The uniformity of specific receptor binding will be assessed by fluorescence microscopy. Binding affinities to both functionalized planar surfaces and vesicles will be determined with radiolabelled proteins.