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Galactose Oxidase - A Radical Enzyme that Catalyzes Radical Reactions to Generate Radical Products

半乳糖氧化酶 - 一种自由基酶，可催化自由基反应产生自由基产物

基本信息

批准号：
9311514
负责人：
Bruce Branchaud
金额：
$ 27万
依托单位：
University of Oregon Eugene
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1993
资助国家：
美国
起止时间：
1993-08-01 至 1997-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9311514&HistoricalAwards=false
关键词：
Galactose Oxidase Radical Enzyme Catalyzes

项目摘要

The wood rot fungus Dactylium dendroides excretes large amounts of the enzyme galactose oxidase (GOase) into the extracellular medium1. GOase has been classified as an alcohol oxidase, using O2 to convert primary alcohols (RCH2OH) into aldehydes (RCHO) and H202. GOase can react with a wide variety of primary alcohols, includingmany carbohydrates, such as glucose, cellobiose, maltose, lactose, sucrose, and polymeric carbohydrates, including cellulose. Although the oxidase reaction of GOase has been known for decades, the main biological purpose of GOase has always been a puzzle. Recent work in the Branchaud laboratory indicates that GOase possesses multiple activities, including an iron reductase activity. Fe(II) produced by the iron reductase activity and H202 from the oxidase activity could combine to produce hydroxyl radicals via the Fenton reaction. The PI proposes that the principle activity of GOase in nature is to generate hydroxyl radicals for the purpose of breaking down wood cellulose and other complex polymers. The research examines and characterizes the multiple activities of this enzyme. %%% Galactose oxidase produces hydroxyl radical, one of the reactive, broad specificity oxidants known. Such a biocatalyst has potential application in oxidatively degrading complex biomolecules in toxic wastes and in developing more environmentally friendly bleaching technologies.

木腐菌Dactyelodendroides分泌大量的半乳糖氧化酶（GOase）进入细胞外中等1. GOase已被归类为醇氧化酶，使用O2 将伯醇（RCH 2OH）转化为醛（RCHO）， H202 GOase可与多种伯醇反应，包括许多碳水化合物，例如葡萄糖、纤维二糖、麦芽糖乳糖、蔗糖和聚合碳水化合物，包括纤维素。虽然GOase的氧化酶反应已经知道了几十年， GOase的主要生物学目的一直是个谜。 Branchaud实验室最近的研究表明，GOase 具有多种活性，包括铁还原酶，活动由铁还原酶活性和H2O2产生的Fe（II）能联合收割机产生羟基自由基通过芬顿反应。 PI建议，天然GOase的主要活性是生成羟基自由基的目的，打破木材纤维素和其他复合聚合物该研究考察并描述了这种酶的多种活性。 %%% 半乳糖氧化酶产生羟基自由基，已知的广泛特异性氧化剂。这种生物催化剂具有潜力在氧化降解有毒复杂生物分子中的应用废物和发展更环保的漂白技术.