Galactose Oxidase - A Radical Enzyme that Catalyzes Radical Reactions to Generate Radical Products

半乳糖氧化酶 - 一种自由基酶，可催化自由基反应产生自由基产物

基本信息

批准号：
9311514
负责人：
Bruce Branchaud
金额：
$ 27万
依托单位：
University of Oregon Eugene
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1993
资助国家：
美国
起止时间：
1993-08-01 至 1997-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9311514&HistoricalAwards=false
关键词：
Galactose Oxidase Radical Enzyme Catalyzes

项目摘要

The wood rot fungus Dactylium dendroides excretes large amounts of the enzyme galactose oxidase (GOase) into the extracellular medium1. GOase has been classified as an alcohol oxidase, using O2 to convert primary alcohols (RCH2OH) into aldehydes (RCHO) and H202. GOase can react with a wide variety of primary alcohols, includingmany carbohydrates, such as glucose, cellobiose, maltose, lactose, sucrose, and polymeric carbohydrates, including cellulose. Although the oxidase reaction of GOase has been known for decades, the main biological purpose of GOase has always been a puzzle. Recent work in the Branchaud laboratory indicates that GOase possesses multiple activities, including an iron reductase activity. Fe(II) produced by the iron reductase activity and H202 from the oxidase activity could combine to produce hydroxyl radicals via the Fenton reaction. The PI proposes that the principle activity of GOase in nature is to generate hydroxyl radicals for the purpose of breaking down wood cellulose and other complex polymers. The research examines and characterizes the multiple activities of this enzyme. %%% Galactose oxidase produces hydroxyl radical, one of the reactive, broad specificity oxidants known. Such a biocatalyst has potential application in oxidatively degrading complex biomolecules in toxic wastes and in developing more environmentally friendly bleaching technologies.

木腐真菌Dactylium dendroides将大量的酶氧化酶（GOASE）排出到细胞外培养基中。山酶已被归类为酒精氧化酶，使用O2将原发性醇（RCH2OH）转化为醛（RCHO）和H202。山酶可以与多种原发性醇反应，包括葡萄糖，纤维糖，麦芽糖，乳糖，蔗糖和聚合物碳水化合物，包括纤维素，包括纤维素，包括纤维素，包括纤维素，包括纤维素。尽管山酶的氧化酶反应数十年来一直闻名，但山酶的主要生物学目的一直是一个难题。 Branchaud实验室的最新工作表明，GOASE具有多种活动，包括铁还原酶活性。铁还原酶活性和来自氧化酶活性的H202产生的Fe（II）可以通过芬顿反应结合起来产生羟基自由基。 PI提出，狗酶在自然界中的原理活性是生成羟基自由基，以分解木纤维素和其他复杂聚合物。该研究检查并表征了该酶的多种活性。 %% %%半乳糖氧化酶产生羟基自由基，这是已知的反应性，广泛的特异性氧化剂之一。这种生物催化剂在有毒废物和开发更环保的漂白技术中氧化降解复杂的生物分子中具有潜在的应用。