Effect of Electroporation on Permeation of Cryoprotectants and Water Across Membranes of Biological Cells

电穿孔对冷冻保护剂和水透过生物细胞膜的影响

• 批准号: 9311906
• 负责人: Ronald Pitt
• 金额: $ 7.58万
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-15 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9311906&HistoricalAwards=false
• 关键词: Effect; Electroporation; Permeation; Cryoprotectants; Water

9311906 Pitt This one year exploratory project is on research to determine the effect of strong electric fields on the permeation of water and cryoprotectants (CPAs) across cell membranes. Membrane permeabilities, as affected by electroporation, will be determined for a model cell system and a single CPA (glycerol) to explore the basic concepts and elucidate mechanisms. This project, which is to provide preliminary data, will involve: (1) the assembly and testing of equipment; (2) a study of the effect of electroporation conditions on cell viability; and (3) experiments to quantify the penetration rate of glycerol into cells at different electroporation conditions. The results of this research may provide important information for the long - term storage of viable cells at cryogenic temperatures. This has applications in biotechnology, agriculture and medicine. ***

这个为期一年的探索性项目是研究确定强电场对水和冷冻保护剂（cpa）穿过细胞膜的渗透的影响。受电穿孔影响的膜透性将被确定为一个模型细胞系统和一个单一的CPA（甘油），以探索基本概念和阐明机制。该项目旨在提供初步数据，将涉及：(1)设备的组装和测试；(2)电穿孔条件对细胞活力影响的研究；(3)实验量化不同电穿孔条件下甘油进入细胞的渗透率。本研究结果可为活细胞在低温下的长期保存提供重要信息。这在生物技术、农业和医学上都有应用。＊＊＊