Effect of Electroporation on Permeation of Cryoprotectants and Water Across Membranes of Biological Cells

小行星9311906 这个为期一年的探索性项目是研究强电场对水和冷冻保护剂（CPA）渗透细胞膜的影响。 膜渗透性，受电穿孔的影响，将被确定为一个模型细胞系统和一个单一的CPA（甘油），以探索基本概念和阐明机制。 该项目旨在提供初步数据，将涉及：（1）设备的组装和测试;（2）研究电穿孔条件对细胞活力的影响;（3）在不同电穿孔条件下量化甘油进入细胞的渗透率的实验。 本研究结果可能为活细胞在低温下的长期保存提供重要信息。 这在生物技术、农业和医学方面都有应用。 ***

9311906 Pitt This one year exploratory project is on research to determine the effect of strong electric fields on the permeation of water and cryoprotectants (CPAs) across cell membranes. Membrane permeabilities, as affected by electroporation, will be determined for a model cell system and a single CPA (glycerol) to explore the basic concepts and elucidate mechanisms. This project, which is to provide preliminary data, will involve: (1) the assembly and testing of equipment; (2) a study of the effect of electroporation conditions on cell viability; and (3) experiments to quantify the penetration rate of glycerol into cells at different electroporation conditions. The results of this research may provide important information for the long - term storage of viable cells at cryogenic temperatures. This has applications in biotechnology, agriculture and medicine. ***