A Molecular Genetic Analysis of Transcription Termination inYeast

酵母转录终止的分子遗传学分析

• 批准号: 9316701
• 负责人: Linda Hyman
• 金额: $ 27.3万
• 依托单位: Tulane University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9316701&HistoricalAwards=false
• 关键词: Molecular; Genetic; Analysis; Transcription; Termination

9316701 Hyman Transcription termination, defined as the release of the nascent transcript and dissociation of RNA polymerase from the template, is arguably the least well understood aspect of eukaryotic transcription. The goal of the proposed research is to contribute to defining the mechanism(s) that are responsible for termination of transcription by RNA polymerase II. There are two specific aims. The first describes experiments that address which sequences are required to direct 3' end formation in vivo. Models proposing that a secondary structure may be important for this event will be tested. The second aim is to utilize an in vitro biochemical assay to complement, and extend the molecular approach. %%% The focus of much research in molecular biology is in the study of gene expression. This is concerned with how genes are turned on in some cell types but not others. This work is concerned with how transcription, an important step in gene expression, is controlled. Specifically we will ask how the enzyme which is responsible for the transcription reaction stops at the appropriate place in the gene. This reaction is referred to as transcription termination. Little is known about transcription termination yet it is an extremely important part of fundamental cell mechanisms. ***

小行星9316701 转录终止，定义为新生转录物的释放和RNA聚合酶从模板上的解离，可以说是真核生物转录中最不为人所知的方面。 拟议研究的目标是有助于定义负责终止RNA聚合酶II转录的机制。 有两个具体目标。 第一个描述了解决体内指导3'末端形成所需的序列的实验。 将对提出二级结构可能对该事件很重要的模型进行测试。 第二个目标是利用体外生化测定来补充和扩展分子方法。 在分子生物学中，许多研究的焦点都集中在基因表达的研究上。 这与基因如何在某些细胞类型中打开而不是其他细胞类型有关。 这项工作是关于如何转录，基因表达的一个重要步骤，是控制。 具体地说，我们将探讨负责转录反应的酶是如何在基因中的适当位置停止的。 该反应被称为转录终止。 关于转录终止知之甚少，但它是基本细胞机制中极其重要的一部分。 ***