A Molecular Genetic Analysis of Transcription Termination inYeast

9316701海曼转录终止，定义为释放新生的转录物和RNA聚合酶从模板上解离，可以说是真核转录中最不为人所知的方面。这项拟议的研究的目标是帮助确定负责RNA聚合酶II终止转录的机制(S)。有两个特定的目标。第一个描述了关于在体内需要哪些序列来指导3‘端形成的实验。提出二级结构对这次活动可能很重要的模型将得到测试。第二个目标是利用体外生化分析来补充，并扩展分子方法。分子生物学的许多研究重点都集中在基因表达的研究上。这与基因在某些类型的细胞中如何启动有关，而与其他类型的细胞无关。这项工作涉及到转录是基因表达中的一个重要步骤，是如何被控制的。具体地说，我们将询问负责转录反应的酶是如何在基因的适当位置停止的。这个反应被称为转录终止。人们对转录终止知之甚少，但它是基本细胞机制中极其重要的一部分。***

9316701 Hyman Transcription termination, defined as the release of the nascent transcript and dissociation of RNA polymerase from the template, is arguably the least well understood aspect of eukaryotic transcription. The goal of the proposed research is to contribute to defining the mechanism(s) that are responsible for termination of transcription by RNA polymerase II. There are two specific aims. The first describes experiments that address which sequences are required to direct 3' end formation in vivo. Models proposing that a secondary structure may be important for this event will be tested. The second aim is to utilize an in vitro biochemical assay to complement, and extend the molecular approach. %%% The focus of much research in molecular biology is in the study of gene expression. This is concerned with how genes are turned on in some cell types but not others. This work is concerned with how transcription, an important step in gene expression, is controlled. Specifically we will ask how the enzyme which is responsible for the transcription reaction stops at the appropriate place in the gene. This reaction is referred to as transcription termination. Little is known about transcription termination yet it is an extremely important part of fundamental cell mechanisms. ***