A Molecular Genetic Analysis of Transcription Termination in Yeast

酵母转录终止的分子遗传学分析

• 批准号: 9604295
• 负责人: Linda Hyman
• 金额: $ 9.9万
• 依托单位: Tulane University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1998-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604295&HistoricalAwards=false
• 关键词: Molecular; Genetic; Analysis; Transcription; Termination

9604295 Hyman Eukaryotic mRNA 3'-end formation occurs by a complex mechanism involving pre-mRNA cleavage and subsequent enzymatic addition of multiple adenosine residues to create a poly(A) tail. Mutations in RNA sequences that direct polyadenylation also result in defects in termination of transcription suggesting that these two processes may be coupled in an as yet undetermined mechanism. Termination of transcription by RNA polymerase II is most likely a complex process involving one or more accessory factors. This research is aimed at understanding how the mechanisms of mRNA polyadenylation and transcription termination converge to form a functional mRNA 3'-end. A yeast model system will take advantage of combined molecular genetic and biochemical approaches to identify trans-acting factors and corresponding genes required for mRNA 3'-end formation and determine their function. The synthesis of a functional eukaryotic messenger RNA requires the formation of a mature mRNA 3'-end, which occurs by a complex mechanism. Mutations in RNA sequences that direct 3'-end formation also result in defects in the termination of transcription suggesting that the two processes may be coupled in an as yet undetermined mechanism. This research is aimed at understanding the mechanistic relationship between mRNA 3'-end formation and transcription termination in a yeast model system.

真核mRNA 3 '末端的形成是通过复杂的机制发生的，包括前体mRNA切割和随后酶促添加多个腺苷残基以产生聚腺苷酸尾。 指导多聚腺苷酸化的RNA序列中的突变也导致转录终止的缺陷，这表明这两个过程可能以尚未确定的机制偶联。 RNA聚合酶II终止转录很可能是一个涉及一个或多个辅助因子的复杂过程。 本研究旨在了解mRNA多聚腺苷酸化和转录终止的机制如何汇聚形成功能性mRNA 3 '端。 酵母模型系统将利用组合的分子遗传学和生物化学方法来鉴定mRNA 3 '端形成所需的反式作用因子和相应的基因并确定它们的功能。 功能性真核生物信使RNA的合成需要成熟mRNA 3 '端的形成，这通过复杂的机制发生。 指导3 '末端形成的RNA序列突变也导致转录终止的缺陷，表明这两个过程可能以尚未确定的机制偶联。本研究旨在了解在酵母模型系统中mRNA 3 '末端形成和转录终止之间的机制关系。