Upgrading the Optical Facilities of the Biology Department at the City College of the City University of New York

纽约城市学院的生物学家正在申请配套资金，以提高- 并为他们的研究提供新的光学设备。要求提供资金， 购买：1）透射电子显微镜（TEM）; 2）倒置荧光显微镜 显微镜;和3）共聚焦激光扫描显微镜。我们还希望提升 一个经常使用的数字扫描电子显微镜（DSEM）的阶段。新的 TEM将取代目前的TEM，21年前购买的菲利普斯300， 作为研究工具的价值有限，因为它不能产生图像 需要回答许多当代研究问题。目前，我们拥有强大的 需要倒置落射荧光显微镜和共聚焦激光扫描 显微镜我们蔡司数码扫描显微镜的现阶段是一个瓶子 #21453;，有效地使用这一工具。现阶段只需要一个标本， 一段时间;我们必须在更换样品之间抽真空。 新舞台有5个小作品 （标本夹）一次。它也是自动化和数字化的。这将允许 研究人员制作蒙太奇。它也将给研究小型生物的研究人员 和细胞，通过存储在存储器中标记感兴趣的样本的位置的能力 这样最好的标本就可以被重新安置、研究和拍照。 由新设备辅助的研究在方面相当广泛，涵盖了 神经生物学、分子发育遗传学、免疫生物学、原生生物学、 分子系统学、内细胞生物学与进化。更具体地 将资助以下项目：1）吞噬体-硅藻中的吞噬溶酶体加工- 有孔虫的核分裂和系统学 有孔虫; 3）浮游硅藻的分子系统学; 4）细胞表面 胸腺滋养细胞的表型; 5）胸腺细胞与胸腺滋养细胞之间的接触 细胞; 6）果蝇复眼的分子和遗传分析; 8） 神经元轴突发育和连接特异性的分子机制 爪蟾的胚胎和变态神经系统;以及 9)信号级联反应中生长因子和神经递质的研究 视网膜调节巩膜生长的手段，当巩膜歪斜时， 导致近视。 城市学院位于纽约市的哈莱姆区。 身体是占主导地位的少数。许多学生参与了提议的 本科生和研究生研究的调查员。如果这项提议得到资助， 人们完全期望少数民族学生将参与研究本身， 以及影响到课程和培训的其他方面。

Biologists at the City College of New York are requesting matching funds to up- gradeand provide new optical facilities for their research. Requested are funds to purchase: 1) a transmission electron microscope (TEM); 2) an inverted epifluorescent microscope; and 3) a confocal laser scanning microscope. We also wish to upgrade the stage of a constantly used digital scanning electron microscope (DSEM).The new TEM will replace the present TEM, a Phillips 300 purchased 21 years ago, which has limited value as a research instrument because it is not capable of producing images required to answer many contemporary research questions. At present we have strong need for an inverted epifluorescent microscope and a confocal laser scanning microscope. The present stage on our Zeiss digital scanning microscope is a bottle neck for effective use of this instrument. The present stage takes only one specimen at a time; we must pump down between specimen changes. The new stage holds 5 stubs (specimen holders) at a time. It also is automated and digitized. This will allow researchers to make montages. It will also give researchers, studying small organisms and cells, the ability to mark positions of specimens of interest by storage in memory so that the best specimens can be relocated, studied, and photographed. The research aided by the new equipment is quite broad in aspect, covering fields such as Neurobiology, Molecular Developmental Genetics, Immunobiology, Protistology, Molecular Systematics, Endocytobiology, and Evolution. More specifically the following projects will be aided: 1) Phagosome- phagolysosomal processing in diatom- bearing endosymbioses in foraminifera; 2)Karyokinesis and systematics of foraminifera; 3) Molecular systematics of planktonic diatoms; 4) Ce ll surface phenotypes of thymic nurse cells; 5) Contact between thymocytes and thymic nurse cells; 6) Molecular and genetic analysis of the Drosophila compound eye; 8) Molecular mechanisms underlying axonal development and connection specificity in the embryonic and metamorphosing nervous system of the frog Xenopus Iclevis; and 9) Studies of growth factors and neurotransmitters involved in the signal cascade by means of which the retina modulates growth of the sclera which, when awry, can lead to myopia. The City College is located in theHarlem section of New York City.and its student body is predominantly minority. Many students are involved with the proposed investigators in undergraduate and graduate research. Should this proposal be funded, there is every expectation that minority students will be involved in the research itself, and the fallout which enters other aspects of coursework and training.