Upgrading the Optical Facilities of the Biology Department at the City College of the City University of New York

纽约市立学院的生物学家正在申请配套资金，以升级并为他们的研究提供新的光学设备。申请资金购买：1)透射电子显微镜（TEM）；2)倒置epifluorescence microscope；3)共聚焦激光扫描显微镜。我们还希望升级一个经常使用的数字扫描电子显微镜（DSEM）的舞台。新的瞬变电磁仪将取代21年前购买的菲利普斯300型瞬变电磁仪，后者作为研究仪器的价值有限，因为它不能产生回答许多当代研究问题所需的图像。目前，我们迫切需要倒置反射荧光显微镜和共聚焦激光扫描显微镜。现阶段，我们的蔡司数码扫描显微镜是一个瓶颈，有效利用这一仪器。现阶段一次只取一个标本；我们必须在标本更换之间进行压缩。新阶段一次可容纳5个存根（标本支架）。它也是自动化和数字化的。这将允许研究人员制作蒙太奇。它还将使研究小生物和细胞的研究人员能够通过存储在记忆中标记感兴趣的标本的位置，以便可以重新定位，研究和拍摄最佳标本。新设备辅助的研究领域相当广泛，涵盖神经生物学、分子发育遗传学、免疫生物学、原生生物学、分子系统学、内吞生物学和进化等领域。更具体地说，以下项目将得到帮助：1)有孔虫中含硅藻内共生的吞噬小体-吞噬溶酶体加工；2)有孔虫的核分裂与分类学；3)浮游硅藻分子系统学；4)胸腺护理细胞的Ce表面表型；5)胸腺细胞与胸腺护理细胞的接触；6)果蝇复眼的分子与遗传分析；8)爪蟾胚胎和变态神经系统轴突发育和连接特异性的分子机制；9)视网膜调节巩膜生长的信号级联过程中涉及的生长因子和神经递质的研究，当巩膜生长发生偏差时，可导致近视。城市学院位于纽约市的哈莱姆区。它的学生主要是少数民族。许多学生在本科和研究生的研究中参与了拟议的调查员。如果这项提案得到资助，少数民族学生将参与到研究中来，并影响到课程和培训的其他方面。

Biologists at the City College of New York are requesting matching funds to up- gradeand provide new optical facilities for their research. Requested are funds to purchase: 1) a transmission electron microscope (TEM); 2) an inverted epifluorescent microscope; and 3) a confocal laser scanning microscope. We also wish to upgrade the stage of a constantly used digital scanning electron microscope (DSEM).The new TEM will replace the present TEM, a Phillips 300 purchased 21 years ago, which has limited value as a research instrument because it is not capable of producing images required to answer many contemporary research questions. At present we have strong need for an inverted epifluorescent microscope and a confocal laser scanning microscope. The present stage on our Zeiss digital scanning microscope is a bottle neck for effective use of this instrument. The present stage takes only one specimen at a time; we must pump down between specimen changes. The new stage holds 5 stubs (specimen holders) at a time. It also is automated and digitized. This will allow researchers to make montages. It will also give researchers, studying small organisms and cells, the ability to mark positions of specimens of interest by storage in memory so that the best specimens can be relocated, studied, and photographed. The research aided by the new equipment is quite broad in aspect, covering fields such as Neurobiology, Molecular Developmental Genetics, Immunobiology, Protistology, Molecular Systematics, Endocytobiology, and Evolution. More specifically the following projects will be aided: 1) Phagosome- phagolysosomal processing in diatom- bearing endosymbioses in foraminifera; 2)Karyokinesis and systematics of foraminifera; 3) Molecular systematics of planktonic diatoms; 4) Ce ll surface phenotypes of thymic nurse cells; 5) Contact between thymocytes and thymic nurse cells; 6) Molecular and genetic analysis of the Drosophila compound eye; 8) Molecular mechanisms underlying axonal development and connection specificity in the embryonic and metamorphosing nervous system of the frog Xenopus Iclevis; and 9) Studies of growth factors and neurotransmitters involved in the signal cascade by means of which the retina modulates growth of the sclera which, when awry, can lead to myopia. The City College is located in theHarlem section of New York City.and its student body is predominantly minority. Many students are involved with the proposed investigators in undergraduate and graduate research. Should this proposal be funded, there is every expectation that minority students will be involved in the research itself, and the fallout which enters other aspects of coursework and training.