MALD-TOF Mass Spectrometer for a Core Laboratory

用于核心实验室的 MALD-TOF 质谱仪

• 批准号: 9318193
• 负责人: Ruth Hogue-Angeletti
• 金额: --
• 依托单位: Yeshiva University, Albert Einstein College of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1996-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9318193&HistoricalAwards=false
• 关键词: MALD; TOF; Mass; Spectrometer; Core

9318193 Hogue-Angeletti We request funds toward the purchase of an instrument for matrix-assisted laser desorption, time-of-flight mass spectrometry (MALD-TOF MS) for a core laboratory with an established record of cooperation and productivity. This instrument will enable a group of investigators at Albert Einstein College of Medicine to determine protein and glycoprotein masses at high sensitivity. In addition to routine analysis of protein samples to assess molecular weight, purity, and to verify structures, the MALD-TOF mass spectrometer will be used to: 1) identify proteins involved in the formation of secretory granules and in protein sorting mechanisms; 2) study the processing of processing enzymes; 3) identify proteins and peptides secreted by ependyma and choroid plexus cells; 4) characterize the taxol binding site on microtubules; S) study the mechanism of ATP- dependent proteases; 6) analysis of proteins rapidly phosphorylated in tyrosine in response to colony stimulating factor-l.

9318193霍格-安吉莱蒂我们要求提供资金，用于购买一台基质辅助激光解吸、飞行时间质谱仪(飞行时间质谱仪)的仪器，用于一个具有既定合作和生产率记录的核心实验室。该仪器将使阿尔伯特·爱因斯坦医学院的一组研究人员能够以高灵敏度测定蛋白质和糖蛋白质量。除了对蛋白质样品进行常规分析以评估相对分子质量、纯度并验证结构外，MALDTOF质谱仪还将用于：1)鉴定与分泌颗粒的形成有关的蛋白质并进行蛋白质分选；2)研究加工酶的处理；3)确认室管膜和脉络丛细胞分泌的蛋白质和多肽；4)确定微管上紫杉醇的结合部位；3)研究三磷酸腺苷依赖的蛋白酶的作用机制；6)分析集落刺激因子L作用下酪氨酸快速磷酸化的蛋白质。