Larval Transcription Factor Mediating Juvenile Hormone Suppression of Genes

9318247琼斯这项研究旨在确定幼虫毛虫发育成有翼成虫现象的分子机制。幼虫形态的维持和成虫形态的抑制，是由于幼虫阶段维持高滴度的保幼激素所致。高滴度的幼虫激素导致与成体相关的基因抑制的分子机制尚不清楚。本研究的目的是以保幼激素抑制六聚体基因表达为模型系统，确定其作用机制。在这项拟议的研究中，我们将分析与六聚体基因相关的调节启动子DNA，以寻找以JH敏感的方式与DNA结合的蛋白质的存在。通过各种测试，包括凝胶迁移率变化分析、DNA足迹和野生型和突变型序列的细胞转染实验，我们将缩小范围并鉴定调控蛋白结合的特定DNA碱基。***

9318247 Jones This research is aimed at determining the molecular mechanisms underlying the phenomenon of development of a larval caterpillar form into the winged, adult moth. The maintenance of a larval form, and the suppression of adult form, is due to the maintenance of a high titer of juvenile hormone during the larval stage. The molecular mechanism by which a high titer of juvenile hormone causes suppression of genes associated with the adult form is not well understood. It is the objective of the present grant research to determine the mechanisms, using as a model system the suppression of expression of hexamerin genes by juvenile hormone. In this proposed research, we will analyze the regulatory promoter DNA associated with the hexamerin genes for the presence of proteins that bind to the DNA in a JH- sensitive manner. By various tests, including gel mobility shift analysis, DNA footprinting and cell transfection assay with wild type and mutant sequences, we will narrow down to and identify the particular DNA bases to which the regulatory proteins bind. ***