Larval Transcription Factor Mediating Juvenile Hormone Suppression of Genes

幼虫转录因子介导保幼激素基因抑制

• 批准号: 9318247
• 负责人: Grace Jones
• 金额: $ 34.98万
• 依托单位: University of Kentucky
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-15 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9318247&HistoricalAwards=false
• 关键词: Larval; Transcription; Factor; Mediating; Juvenile

9318247 Jones This research is aimed at determining the molecular mechanisms underlying the phenomenon of development of a larval caterpillar form into the winged, adult moth. The maintenance of a larval form, and the suppression of adult form, is due to the maintenance of a high titer of juvenile hormone during the larval stage. The molecular mechanism by which a high titer of juvenile hormone causes suppression of genes associated with the adult form is not well understood. It is the objective of the present grant research to determine the mechanisms, using as a model system the suppression of expression of hexamerin genes by juvenile hormone. In this proposed research, we will analyze the regulatory promoter DNA associated with the hexamerin genes for the presence of proteins that bind to the DNA in a JH- sensitive manner. By various tests, including gel mobility shift analysis, DNA footprinting and cell transfection assay with wild type and mutant sequences, we will narrow down to and identify the particular DNA bases to which the regulatory proteins bind. ***

9318247 Jones这项研究的目的是确定毛虫幼虫发育为有翼的成虫的分子机制。幼虫形态的维持和成虫形态的抑制是由于在幼虫阶段维持了高滴度的幼虫激素。高滴度的幼虫激素导致与成虫形式相关的基因抑制的分子机制尚不清楚。本研究的目的是确定其机制，以幼激素抑制hexamerin基因的表达为模型系统。在这项拟议的研究中，我们将分析与六聚氰胺基因相关的调控启动子DNA是否存在以JH敏感方式结合到DNA上的蛋白质。通过各种测试，包括凝胶迁移位移分析，DNA足迹和野生型和突变序列的细胞转染试验，我们将缩小并确定调节蛋白结合的特定DNA碱基。＊＊＊